PMID: 11922897Mar 30, 2002Paper

Reduced swelling-activated Cl(-) current densities in hypertrophied ventricular myocytes of rabbits with heart failure

Cardiovascular Research
Marcel M G J van BorrenJ H Ravesloot

Abstract

Hypertrophied myocytes of failing hearts have prolonged action potential durations. It is unknown how the swelling-activated Cl(-) current (I(Cl,swell)) affects the abnormal AP configuration. We studied I(Cl,swell) in ventricular myocytes isolated from failing and age-matched normal rabbit hearts. We applied whole-cell patch-clamp methodology and activated I(Cl,swell) by lowering tonicity of the superfusate. Neither with ruptured-patch nor with amphotericin B perforated-patch, whole-cell clamp we found I(Cl,swell) active under isotonic conditions in either the normal or the hypertrophied failing heart (HFH) myocytes. I(Cl,swell) caused AP shortening and resting membrane potential (V(m)) depolarization in an osmotic gradient-dependent fashion. However, in the HFH myocytes swelling-induced AP changes were significantly smaller, even though the cells underwent the same relative change in planar cell surface area. Voltage-clamp experiments revealed that in HFH myocytes I(Cl,swell) current density was approximately 50% reduced. Reduced I(Cl,swell) densities in HFH myocytes cause limited AP shortening and V(m) depolarization upon swelling of the cells.

Citations

May 24, 2011·Acta Pharmacologica Sinica·Dayue Darrel Duan
Jul 10, 2013·Journal of Cardiovascular Translational Research·Erik BlaauwFrans A van Nieuwenhoven
Jul 16, 2011·Journal of Pharmacological Sciences·Shintaro YamamotoTakahiro Iwamoto
May 14, 2004·European Journal of Pharmacology·Lesya M ShubaTerence F McDonald
Nov 7, 2015·Reproductive Sciences·Melissa MarianaIgnacio Verde
Jul 30, 2003·Circulation·Arie O VerkerkE Etienne Verheijck
Jun 21, 2007·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·Arie O VerkerkHanno L Tan
Apr 28, 2019·International Journal of Molecular Sciences·Arie O VerkerkRonald Wilders
Aug 27, 2021·Frontiers in Cardiovascular Medicine·Daniel TurnerRajan Sah

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