Reduced ventromedial hypothalamic neuronal nitric oxide synthase and increased sensitivity to NOS inhibition in dietary obese rats: further evidence of a role for nitric oxide in the regulation of energy balance

Brain Research
C J Sadler, J P H Wilding

Abstract

Inhibition of hypothalamic nitric oxide (NO) decreases energy intake, and changes in hypothalamic NO synthase (NOS) have been observed in genetically obese rodents, but it is not known if NO is involved in the development of diet-induced obesity (DIO). We therefore measured changes in hypothalamic neuronal NOS (nNOS) in DIO and investigated effects of peripheral and central inhibition of NOS in this model. Expression of nNOS in relation to changes in nutritional state was measured by immunohistochemistry, with radiochemical detection. The effect of chronic intraperitoneal (i.p.) administration of the NOS inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME, 50 mg/kg/day) on energy intake, bodyweight and hypothalamic nitric oxide content was assessed in both chow-fed and DIO animals. Twenty-four hour energy intake after acute intracerebroventricular (i.c.v.) of L-NAME was also measured. Diet-induced obese animals had a statistically significant 32% reduction in the number of nNOS-immunolabelled cells in the ventromedial hypothalamus compared to chow-fed controls. Intraperitoneal administration of L-NAME decreased hypothalamic NO content in both chow-fed and DIO. Energy intake was reduced by 16% in DIO over 16 days, whereas energ...Continue Reading

References

Sep 6, 1979·Nature·N J Rothwell, M J Stock
Jan 1, 1991·Life Sciences·J E Morley, J F Flood
Jan 16, 1989·Biochemical and Biophysical Research Communications·R M Palmer, S Moncada
May 10, 1996·Neuroscience Letters·J E Morley, M B Mattammal
Dec 31, 1997·Obesity Research·K A al-BarazanjiI S Gloger
Jul 25, 2000·Diabetes·J F GautierP A Tataranni
Mar 16, 2001·Progress in Neurobiology·H Prast, A Philippu
Jul 31, 2002·Diabetic Medicine : a Journal of the British Diabetic Association·J P H Wilding
Jul 2, 2003·Journal of Neuroendocrinology·S O FetissovT Hökfelt
Oct 10, 2003·American Journal of Physiology. Endocrinology and Metabolism·Susan J Vannucci, Ian A Simpson

❮ Previous
Next ❯

Citations

Mar 27, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Carrie A NorthcottJoseph R Haywood
Dec 24, 2015·Current Hypertension Reports·Marisa Nile MolinaWalter Manucha
May 12, 2012·Progress in Neurobiology·Abraham J Susswein, Hillel J Chiel
Jun 1, 2014·Free Radical Biology & Medicine·Brian E Sansbury, Bradford G Hill
Jul 6, 2016·Clínica e investigación en arteriosclerosis : publicación oficial de la Sociedad Española de Arteriosclerosis·Alejandra B Camargo, Walter Manucha
Jun 30, 2018·Biotechnology and Applied Biochemistry·Anastasija PanicEsma R Isenovic
Apr 18, 2009·The Australian and New Zealand Journal of Psychiatry·Michael RobertsonGarry Walter
Dec 5, 2008·Child: Care, Health and Development·S N LewisUNKNOWN Families, Children and Child Care (FCCC) project team
Jan 10, 2009·Family Process·Elizabeth A CannonMargaret Szewczyk Sokolowski
Feb 21, 2009·Child: Care, Health and Development·A F WestUNKNOWN FCCC project team
Aug 4, 2009·Child: Care, Health and Development·Phillip R Sevigny, L Loutzenhiser
Dec 17, 2009·Birth·Lucia Genesoni, Maria Anna Tallandini
Sep 1, 2010·Personal Relationships·Catherine K Buckley, Sarah J Schoppe-Sullivan
Jul 22, 2011·Family Relations·Sandra L Hofferth, Frances Goldscheider
Nov 1, 2010·Infant Mental Health Journal·Naama Atzaba-PoriaBaruch Yerushalmi
Jun 1, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Debra D CanabalVanessa H Routh
Jan 21, 2010·American Journal of Community Psychology·Jessica Ball
Jan 5, 2013·Personal Relationships·Jason T Castillo, Christian M Sarver
Jul 31, 2012·Journal of Marriage and the Family·Jennifer L Hook
Jul 2, 2014·Journal of Child Psychology and Psychiatry, and Allied Disciplines·Catherine Panter-BrickJames F Leckman
Jan 29, 2011·Journal of Neurophysiology·N MillerA J Susswein
Jul 5, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Meredith B Barnes, J Lee Beverly

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.