Reducing adsorption to improve recovery and in vivo detection of neuropeptides by microdialysis with LC-MS

Analytical Chemistry
Ying ZhouRobert T Kennedy

Abstract

Neuropeptides are an important class of neurochemicals; however, measuring their concentration in vivo by using microdialysis sampling is challenging due to their low concentration and the small samples generated. Capillary liquid chromatography with mass spectrometry (cLC-MS) can yield attomole limits of detection (LOD); however, low recovery and loss of sample to adsorptive surfaces can still hinder detection of neuropeptides. We have evaluated recovery during sampling and transfer to the cLC column for a selection of 10 neuropeptides. Adding acetonitrile to sample eliminated carryover and improved LOD by 1.4- to 60-fold. The amount of acetonitrile required was found to have an optimal value that correlated with peptide molecular weight and retention time on a reversed phase LC column. Treating AN69 dialysis membrane, which bears negative charge due to incorporated sulfonate groups, with polyethylenimine (PEI) improved recovery by 1.2- to 80-fold. The effect appeared to be due to reducing electrostatic interaction between peptides and the microdialysis probe because modification increased recovery only for peptides that carried net positive charge. The combined effects improved LOD of the entire method by 1.3- to 800-fold for...Continue Reading

References

Dec 1, 1980·Journal of Neuroscience Methods·G PaxinosP C Emson
Jun 11, 1998·Peptides·C L NilssonR Ekman
Feb 22, 2000·Journal of Drug Targeting·G F LemkineB A Demeneix
Nov 7, 2000·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·G YadidJ Sagen
Sep 8, 2001·Experimental Brain Research·K Hygge BlakemanP Alster
Oct 3, 2002·Journal of Neurochemistry·Glen R HansonMario E Alburges
Sep 19, 2003·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Sylvie LavaudJacques Chanard
Feb 6, 2004·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Holger GrohganzMartin Brandl
Feb 3, 2007·Journal of Proteome Research·Paul M van MidwoudHarm A G Niederländer
Aug 11, 2007·Journal of Separation Science·Katrien LanckmansYvette Michotte
Jun 23, 2009·Analytical Chemistry·Goran MitulovićKarl Mechtler
Jun 25, 2009·Pharmacological Reviews·Natsuko Tsujino, Takeshi Sakurai
May 24, 2011·Journal of Neuroscience Methods·Anthony W Herbaugh, Julie A Stenken
Aug 26, 2011·Contributions to Nephrology·Michel ThomasIngrid Ledebo
Dec 14, 2011·Analytical and Bioanalytical Chemistry·Andreas P DahlinMagnus Wetterhall
Jun 27, 2012·Journal of Neuroscience Methods·Ramakrishna NirogiDevender Reddy Ajjala
Sep 25, 2012·Current Biology : CB·Alexandra G DiFeliceantonioKent C Berridge
Apr 18, 2013·Journal of the American Society for Mass Spectrometry·Ying ZhouRobert T Kennedy

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Citations

Sep 4, 2018·ELife·Ream Al-HasaniMichael R Bruchas
Jul 1, 2017·The AAPS Journal·Alexander G Zestos, Robert T Kennedy
Jan 27, 2019·CPT: Pharmacometrics & Systems Pharmacology·Willem J van den BrinkElizabeth C M de Lange
Dec 16, 2020·Scientific Reports·Alexandra HögstedtSimon Farnebo
Mar 3, 2020·Brain Research·Anushree N Karkhanis, Ream Al-Hasani
Jan 21, 2017·Analytical Chemistry·Mallikarjunarao GanesanaB Jill Venton
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lukasz ChrobokMarian Henryk Lewandowski
Sep 25, 2021·Mass Spectrometry Reviews·Ashley PhetsanthadLingjun Li

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