Reducing isozyme competition increases target fatty acid accumulation in seed triacylglycerols of transgenic Arabidopsis

Plant Physiology
Harrie van ErpJ. Browse

Abstract

One goal of green chemistry is the production of industrially useful fatty acids (FAs) in crop plants. We focus on hydroxy fatty acids (HFAs) and conjugated polyenoic FAs (α-eleostearic acids [ESAs]) using Arabidopsis (Arabidopsis thaliana) as a model. These FAs are found naturally in seed oils of castor (Ricinus communis) and tung tree (Vernicia fordii), respectively, and used for the production of lubricants, nylon, and paints. Transgenic oils typically contain less target FA than that produced in the source species. We hypothesized that competition between endogenous and transgenic isozymes for substrates limits accumulation of unique FAs in Arabidopsis seeds. This hypothesis was tested by introducing a mutation in Arabidopsis diacylglycerol acyltransferase1 (AtDGAT1) in a line expressing castor FA hydroxylase and acyl-Coenzyme A:RcDGAT2 in its seeds. This led to a 17% increase in the proportion of HFA in seed oil. Expression of castor phospholipid:diacylglycerol acyltransferase 1A in this line increased the proportion of HFA by an additional 12%. To determine if our observations are more widely applicable, we investigated if isozyme competition influenced production of ESA. Expression of tung tree FA conjugase/desaturase in...Continue Reading

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Citations

Apr 9, 2016·International Journal of Molecular Sciences·Grace Q ChenJiann-Tsyh Lin
Apr 27, 2017·Plant & Cell Physiology·J A Aznar-Moreno, T P Durrett
Jan 14, 2017·Plant Biotechnology Journal·Olga YurchenkoJohn M Dyer
Mar 5, 2018·Applied Microbiology and Biotechnology·Roman HolicGuanqun Chen
Oct 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Harrie van ErpPeter J Eastmond
Nov 18, 2017·Plant & Cell Physiology·Daniel LunnJohn Browse
Jun 24, 2020·The Plant Journal : for Cell and Molecular Biology·Yongtai YinMaoteng Li
Oct 2, 2020·The Plant Journal : for Cell and Molecular Biology·Sandra M CorreaZoran Nikoloski
Aug 19, 2017·Plant Science : an International Journal of Experimental Plant Biology·Jose A Aznar-Moreno, Timothy P Durrett
Mar 2, 2019·Progress in Lipid Research·Thomas VanherckeSurinder P Singh
Apr 23, 2020·Journal of Agricultural and Food Chemistry·Zheng YangMeng Zhang
Jul 9, 2020·Progress in Lipid Research·Sandra M CorreaYariv Brotman
Feb 24, 2021·The Plant Journal : for Cell and Molecular Biology·Linah AlkotamiTimothy P Durrett
Jun 7, 2021·Metabolic Engineering·Harrie van ErpPeter J Eastmond

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