Reduction and expansion of the glutamine synthetase expressing zone in livers from tetracycline controlled TGF-beta1 transgenic mice and multiple starved mice

Journal of Hepatology
Elke UeberhamR Gebhardt

Abstract

To learn more about tissue remodelling in fibrotic livers of tetracycline-controlled TGF-beta1 transgenic mice (TGF-beta1-on-mice) and during regeneration after removal of the fibrotic stimulus (off-mice), we investigated the expression of glutamine synthetase (GS), an exclusive pericentrally expressed enzyme. GS was localised immunohistochemically and quantified by real-time RT-PCR and enzymatic activity measurement. Apoptosis in livers of TGF-beta1-on-mice was demonstrated by in situ apoptosis detection kit (TUNEL reaction). Livers of TGF-beta1-on-mice harbour a reduced number of GS-positive hepatocytes and expression of GS is downregulated, while multiple starved mice serving as controls for malnutrition during TGF-beta1 exposure surprisingly showed an impressive amplification of GS-positive hepatocytes. Apoptotic events were frequent around central veins in livers of TGF-beta1-on-mice, while in multiple induced mice apoptosis was dominant around all vessels and weak in midzonal areas. During regeneration from fibrosis, control levels were regained within 21 days. Beta-catenin was dislocated from plasma membrane to cytoplasm exclusively in pericentral hepatocytes during a short time slot after a unique expression of TGF-beta...Continue Reading

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Citations

Jul 3, 2007·Journal of Molecular Histology·Elke UeberhamRolf Gebhardt
Aug 9, 2011·Journal of Molecular Histology·Sandra SchreiberChristoph Köhle
Oct 15, 2010·Comparative Hepatology·Elke UeberhamRolf Gebhardt
Aug 13, 2015·International Journal of Molecular Sciences·Dong Hwan KimKyu-Shik Jeong
Dec 7, 2007·Journal of Cellular and Molecular Medicine·Elke UeberhamUwe Ueberham
Oct 7, 2015·Archives of Toxicology·Martin BartlPu Li
Oct 28, 2016·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Gillian HaleSanjay Kakar
May 10, 2008·Journal of Hepatology·Sandra RebouissouJessica Zucman-Rossi
Jul 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rolf Gebhardt, Elke Ueberham
Nov 5, 2014·Liver International : Official Journal of the International Association for the Study of the Liver·Olivia Vázquez-MartínezMauricio Díaz-Muñoz
Jun 14, 2019·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Nancy M JosephSanjay Kakar
Mar 21, 2007·Archives of Biochemistry and Biophysics·Jacek LenartThomas Kietzmann

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