Reduction in activating transcription factor 4 promotes carbon tetrachloride and lipopolysaccharide/D‑galactosamine‑mediated liver injury in mice

Molecular Medicine Reports
Xiaofang ZhaoRongyang Dai

Abstract

Although activating transcription factor 4 (ATF4) is involved in the regulation of numerous biological functions, whether ATF4 has a direct role in liver injury is unknown. The aim of the present study was to investigate the role of ATF4 in liver injury using mouse models. The results revealed that ATF4 protein is expressed markedly higher in the mouse liver when in comparison with other tissues. Notably, tunicamycin treatment, an endoplasmic reticulum (ER) stress inducer, induced the phosphorylation of eukaryotic translation initiation factor 2α (eIF2α), but decreased ATF4 protein levels in the mouse liver. This suggested an unconventional regulation pattern of ATF4 protein not associated with ER stress or eIF2α. In addition, it was also observed that the liver levels of ATF4 protein were significantly reduced upon chronic liver injury induced by carbon tetrachloride (CCl4). ATF4 protein was also decreased in acute liver injury induced by lipopolysaccharide (LPS) plus D‑galactosamine (D‑GalN). Furthermore, the results revealed that knockdown of ATF4 by injecting ATF4‑targeting Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)‑CRISPR associated protein 9 plasmids exacerbated CCl4 and LPS/D‑GalN‑induced liver in...Continue Reading

References

Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B A KarpinskiJ M Leiden
Mar 10, 2001·Molecular and Cellular Biology·I LassotF Margottin-Goguet
Jun 8, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T NakamaH Tsubouchi
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 12, 2003·Developmental Cell·D Thomas Rutkowski, Randal J Kaufman
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Krishna M Vattem, Ronald C Wek
Oct 13, 2005·The Journal of Biological Chemistry·Irina LassotFlorence Margottin-Goguet
Mar 16, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Robert F Schwabe, David A Brenner
May 7, 2008·The Journal of Experimental Medicine·Philipp S LangeRajiv R Ratan
Sep 4, 2009·The Journal of Clinical Investigation·Tatsuya YoshizawaGerard Karsenty
Oct 6, 2009·Trends in Endocrinology and Metabolism : TEM·Michael S KilbergNan Su
Jan 13, 2010·Cell Research·Chunxia WangFeifan Guo
Mar 17, 2011·Genes & Development·Madhumita DasRoger J Davis
Jun 8, 2011·The Biochemical Journal·Houkai LiFeifan Guo
Mar 2, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·David Y Zhang, Scott L Friedman
Jul 28, 2013·The Journal of Biological Chemistry·Guozhi XiaoH Henry Dong
Jan 1, 2014·Journal of Cellular and Molecular Medicine·Chunxia WangFeifan Guo
Feb 1, 2014·The American Journal of Pathology·Xin Chen, Diego F Calvisi
May 13, 2014·Biological & Pharmaceutical Bulletin·Xing LinQuanfang Huang
Sep 12, 2015·Journal of Clinical and Translational Hepatology·Bénédicte DelireIsabelle Leclercq
Sep 28, 2017·Mediators of Inflammation·Yachao TaoHong Tang

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.