Reduction in duodenal endocrine cells in irritable bowel syndrome is associated with stem cell abnormalities

World Journal of Gastroenterology : WJG
M El-SalhyTrygve Hausken

Abstract

To determine whether the decreased density of duodenal endocrine cells in irritable bowel syndrome (IBS) is associated with abnormalities in stem cell differentiation. The study sample comprised 203 patients with IBS (180 females and 23 males with a mean age of 36 years) and a control group of 86 healthy subjects without gastrointestinal complaints (77 females and 9 males with a mean age of 38 years). The patients included 80 with mostly diarrhoea (IBS-D), 47 with both diarrhoea and constipation (IBS-M), and 76 with mostly constipation (IBS-C). Both the patients and controls underwent gastroscopy and four biopsy samples were taken from the descending part of the duodenum, proximal to the papilla of Vater. The biopsy samples were sectioned and immunostained for Musashi 1 (Msi-1), neurogenin 3 (NEUROG3), secretin, cholecystokinin (CCK), gastric inhibitory peptide (GIP), somatostatin and serotonin. Immunostaining was performed with an ultraView Universal DAB Detection Kit (v1.02.0018, Venata Medical Systems, Basal, Switzerland) using the BenchMark Ultra immunohistochemistry/in situ hybridization staining module (Venata Medical Systems). Endocrine cell densities were quantified by computerized image analysis using the Olympus cellS...Continue Reading

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Citations

Jul 15, 2015·Expert Review of Gastroenterology & Hepatology·Magdy El-Salhy
Dec 9, 2016·Expert Review of Gastroenterology & Hepatology·Magdy El-SalhyJan Gunnar Hatlebakk
Jul 4, 2018·Journal of Neurogastroenterology and Motility·Hirokazu FukuiHiroto Miwa
Jul 15, 2015·World Journal of Gastroenterology : WJG·Magdy El-Salhy
Oct 16, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dervla O'Malley
Nov 17, 2017·World Journal of Gastroenterology : WJG·Samuel O IgbinedionJonathan S Alexander
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Aug 26, 2020·Journal of Gastroenterology and Hepatology·Ko MiuraHiroto Miwa
Jul 25, 2017·Experimental Biology and Medicine·Tarek Mazzawi, Magdy El-Salhy
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Feb 11, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Justin BrandlerMichael Camilleri
Apr 21, 2020·World Journal of Gastroenterology : WJG·Magdy El-Salhy
Dec 22, 2017·Signal Transduction and Targeted Therapy·Xudong ZhuQinsong Sheng

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