Reduction in horizontal transfer of conjugative plasmid by UV irradiation and low-level chlorination

Water Research
Wenfang LinXin Yu

Abstract

The widespread presence of antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARB) in the drinking water system facilitates their horizontal gene transfer among microbiota. In this study, the conjugative gene transfer of RP4 plasmid after disinfection including ultraviolet (UV) irradiation and low-level chlorine treatment was investigated. It was found that both UV irradiation and low-level chlorine treatment reduced the conjugative gene transfer frequency. The transfer frequency gradually decreased from 2.75 × 10(-3) to 2.44 × 10(-5) after exposure to UV doses ranging from 5 to 20 mJ/cm(2). With higher UV dose of 50 and 100 mJ/cm(2), the transfer frequency was reduced to 1.77 × 10(-6) and 2.44 × 10(-8). The RP4 plasmid transfer frequency was not significantly affected by chlorine treatment at dosages ranging from 0.05 to 0.2 mg/l, but treatment with 0.3-0.5 mg/l chlorine induced a decrease in conjugative transfer to 4.40 × 10(-5) or below the detection limit. The mechanisms underlying these phenomena were also explored, and the results demonstrated that UV irradiation and chlorine treatment (0.3 and 0.5 mg/l) significantly reduced the viability of bacteria, thereby lowering the conjugative transfer frequency...Continue Reading

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Citations

Aug 24, 2019·Nature Microbiology·Sara Hernando-AmadoJosé L Martínez
Mar 7, 2020·Frontiers of Environmental Science & Engineering·Virender K SharmaMingbao Feng
Nov 25, 2018·International Journal of Environmental Research and Public Health·Ewa Brągoszewska, Izabela Biedroń
Sep 29, 2020·Frontiers in Microbiology·Sara Hernando-AmadoJosé L Martínez
Dec 3, 2020·International Journal of Environmental Research and Public Health·Gabriela BairánEduardo Torres
Nov 7, 2020·Environmental Science and Pollution Research International·Polina GalitskayaSvetlana Selivanovskaya
Oct 11, 2020·Environment International·Hao ZhengBaoshan Xing

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