Reduction in mutation frequency by very low-dose gamma irradiation of Drosophila melanogaster germ cells

Radiation Research
Keiji OguraTakao Koana

Abstract

To determine whether the linear no-threshold (LNT) model for stochastic effects of ionizing radiation is applicable to very low-dose radiation at a low dose rate, we irradiated immature male germ cells of the fruit fly, Drosophila melanogaster, with several doses of (60)Co gamma rays at a dose rate of 22.4 mGy/h. Thereafter, we performed the sex-linked recessive lethal mutation assay by mating the irradiated males with nonirradiated females. The mutation frequency in the group irradiated with 500 microGy was found to be significantly lower than that in the control group (P < 0.01), whereas in the group subjected to 10 Gy irradiation, the mutation frequency was significantly higher than that in the control group (P < 0.03). A J-shaped dose-response relationship was evident. Molecular experiments using DNA microarray and quantitative reverse transcription PCR indicated that several genes known to be expressed in response to heat or chemical stress and grim, a positive regulator of apoptosis, were up-regulated immediately after irradiation with 500 microGy. The involvement of an apoptosis function in the non-linear dose-response relationship was suggested.

References

Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Y KawataH Ishikawa
Jan 1, 1987·Journal of Cell Science. Supplement·J B BoydK Sakaguchi
Jul 1, 1995·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·C S WongR P Hill
May 13, 1999·Science·A B HoutsmullerW Vermeulen
Jul 3, 2002·The EMBO Journal·Cristina ClaveríaMiguel Torres
Apr 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kai Rothkamm, Markus Löbrich
Aug 5, 2003·Molecular and Cellular Biology·Suzanne RademakersWim Vermeulen
Dec 19, 1958·Science·W L RUSSELLE M KELLY
Jan 20, 2004·Molecular and Cellular Biology·Michael H BrodskyGerald M Rubin
May 26, 2005·Radiation Research·Noreli FrancoMichèle T Martin
Jun 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Anita WichmannTin Tin Su
Aug 1, 1987·Molecular & General Genetics : MGG·Y H Inoue, M T Yamamoto

❮ Previous
Next ❯

Citations

May 20, 2011·Health Physics·Bobby Scott
Nov 3, 2015·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Shizuyo Sutou
Jul 24, 2013·Critical Reviews in Toxicology·Edward J Calabrese
Jun 29, 2016·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Shizuyo Sutou
Jan 11, 2013·Dose-response : a Publication of International Hormesis Society·Mohan Doss
Dec 4, 2013·Dose-response : a Publication of International Hormesis Society·Jerry M Cuttler
Jan 2, 2015·Dose-response : a Publication of International Hormesis Society·Michael AntoshLeon N Cooper
Oct 18, 2014·Journal of Cell Communication and Signaling·Bobby R Scott
Jun 15, 2016·International Journal of Cancer. Journal International Du Cancer·Guozi YangJiuwei Cui
Apr 1, 2017·Cancer Immunology, Immunotherapy : CII·Marek K JaniakEdward J Calabrese
Mar 25, 2011·Dose-response : a Publication of International Hormesis Society·Jerry M Cuttler
Jan 11, 2013·Dose-response : a Publication of International Hormesis Society·Charles L Sanders
Jan 1, 2011·Dose-response : a Publication of International Hormesis Society·Bobby R Scott
Sep 30, 2010·Dose-response : a Publication of International Hormesis Society·Jerry M Cuttler
Oct 20, 2018·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Shizuyo SutouUNKNOWN Mammalian Mutagenicity Study Group
Apr 28, 2020·International Journal of Radiation Biology·Luz Ma VidalJuan C Sánchez-Meza
Jul 30, 2016·Health Physics·Jerry M Cuttler
Jul 18, 2018·Dose-response : a Publication of International Hormesis Society·John J Cardarelli, Brant A Ulsh
Apr 20, 2019·Dose-response : a Publication of International Hormesis Society·L DobrzyńskiM K Janiak
May 15, 2015·American Journal of Physiology. Endocrinology and Metabolism·Minglong ShaoLu Cai
Jan 2, 2019·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Shizuyo Sutou
Nov 25, 2020·Environmental and Molecular Mutagenesis·Elena Yushkova, Ludmila Bashlykova
Dec 9, 2020·Journal of Radiation Research·Yoshiharu Tanaka, Masakazu Furuta

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE9552
GSM241755
GSM241769

Methods Mentioned

BETA
irradiate
Chip
X-ray

Software Mentioned

Excel Addin Macro Statcel2
GeneChip Operating

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis