Reduction in nonparenchymal cell injury and vascular endothelial dysfunction after cold preservation of the liver by gaseous oxygen

Transplant International : Official Journal of the European Society for Organ Transplantation
T MinorH Klauke

Abstract

Reintroduction of oxygen to previously anoxic tissue may result in severe cell injury (oxygen paradox) and contribute to the so-called reperfusion damage of ischemic organs. Our study investigated the influence of simple gaseous oxygen supply during ischemia on nonparenchymal cell alterations upon reperfusion of the liver. Livers from male Wistar rats were isolated, rinsed blood-free and stored for 48 h at 4 degrees C in UW-preservation solution (group 1; n = 6). Gaseous oxygen was insufflated into a second group of livers (group 2; n = 6) during the storage period via the inferior caval vein at a pressure limited to 18 mmHg. To simulate the period of slow rewarming of the organ during surgical implantation in vivo, all livers were incubated at 25 degrees C in saline solution for 30 min prior to reperfusion. Reperfusion was carried out in vitro in a recirculating system with Krebs-Henseleit buffer. A control group was perfused immediately after harvest. The technique of aerobic storage (group 2) resulted in normal vascular perfusion characteristics without elevation of portal venous pressure (PVP) above control values, in contrast to group 1 livers which showed a significantly elevated PVP, averaging between 1.5 and 2 times the...Continue Reading

References

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Citations

Feb 4, 2012·Cryobiology·Thomas M SuszynskiKlearchos K Papas
Sep 21, 2010·The Journal of Surgical Research·Martina KoettingThomas Minor
Sep 13, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·T MinorA Paul
Mar 19, 2014·Transplant International : Official Journal of the European Society for Organ Transplantation·Mettu S ReddyDavid Talbot
Apr 29, 2014·Journal of Hepatology·Jay A Graham, James V Guarrera
Dec 18, 2013·International Reviews of Immunology·Mihai Oltean, Thomas A Churchill
Apr 6, 2018·Pancreas·Hirotake KomatsuYoko Mullen
May 12, 2001·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·M Kukan, P S Haddad
Jul 1, 2016·World Journal of Transplantation·Paria MahboubPaulo N Martins
Aug 22, 2006·Transplant International : Official Journal of the European Society for Organ Transplantation·Christoph HeidenhainPeter Neuhaus
Feb 29, 2008·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Jürgen TreckmannAndreas Paul
Mar 21, 1998·Transplant International : Official Journal of the European Society for Organ Transplantation·T MinorW Isselhard

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