Reduction in porphyrin excretion as a sensitive indicator of lead toxicity in primary cultures of adult rat hepatocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
B Quintanilla-VegaT Mendoza-Figueroa

Abstract

Alterations of specific metabolic pathways can be used as sensitive indicators of toxicity by chemicals and can give valuable information on the mechanism(s) involved. Short-term effects of lead on hepatic haem biosynthesis were studied in an in vitro system. Primary cultures of adult rat hepatocytes were exposed for 24-48 hr to lead (0.024-3.6 mm), and excreted and intracellular porphyrins were measured in untreated and lead-treated cultures. Cytotoxicity, as estimated by enzyme leakage, and morphological alterations were also evaluated. Control hepatocytes produced porphyrins at a rate of 387 pmol/mg cellular protein/day. Most of the released and intracellular porphyrins were protoporphyrins, although uro- and coproporphyrins were also detected in lower amounts. After 24 hr of exposure to 0.1-3.6 mm Pb(2+) , excreted porphyrins decreased by 24-92% and intracellular porphyrins by 36-60%, while 48 hr of exposure to 0.024-3.6 mm Pb(2+) caused a progressive reduction of 77-97% in porphyrin excretion and of 49-67% in intracellular porphyrins. Lead exposure also produced a differential decrease of proto-, copro- and uro-porphyrin excretion. These lead effects can be explained mainly by inhibition of the enzyme 5-aminolaevulinate de...Continue Reading

References

Aug 1, 1989·Differentiation; Research in Biological Diversity·W Kuri-Harcuch, T Mendoza-Figueroa
Nov 15, 1988·The Biochemical Journal·P R SinclairU Muller-Eberhard
Jan 1, 1987·Annals of the New York Academy of Sciences·A Bernard, R Lauwerys
Jan 1, 1987·Annals of the New York Academy of Sciences·J S Woods, B A Fowler
Feb 1, 1983·European Journal of Biochemistry·R Aguirre, V Kasche
Jul 1, 1957·American Journal of Clinical Pathology·S REITMAN, S FRANKEL
Apr 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A Sierra-SantoyoT Mendoza-Figueroa

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Citations

Oct 18, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K Zierold
Feb 20, 2015·Biological Trace Element Research·V M AndradeA P Marreilha dos Santos
Aug 27, 2021·Toxicology and Applied Pharmacology·V Lopes de AndradeA P Marreilha Dos Santos

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