PMID: 3768304Aug 12, 1986Paper

Reduction kinetics of the ferredoxin-ferredoxin-NADP+ reductase complex: a laser flash photolysis study

Biochemistry
A K BhattachryyaG Tollin

Abstract

The kinetics of reduction of spinach ferredoxin (Fd), ferredoxin-NADP+ reductase (FNR), and the Fd-FNR complex have been investigated by the laser flash photolysis technique. 5-Deazariboflavin semiquinone (5-dRf), generated in situ by laser flash photolysis under anaerobic conditions, rapidly reduced both oxidized Fd (Fdox) (k = 2 X 10(8) M-1 s-1) and oxidized FNR (FNRox) (K = 6.3 X 10(8) M-1 s-1) at low ionic strength (10 mM) at pH 7.0, leading to the formation of reduced Fd (Fdred) and FNR semiquinone (FNR.), respectively. At higher ionic strengths (310 and 460 mM), the rate constant for the reduction of the free Fdox increased about 3-fold (k = 6.7 X 10(8) M-1 s-1 at 310 mM and 6.4 X 10(8) M-1 s-1 at 460 mM). No change in the second-order rate constant for reduction of the free FNRox was observed at high ionic strength. At low ionic strength (10 mM), 5-dRf. reacted only with the FAD center of the preformed 1:1 Fdox-FNRox complex (k = 5.6 X 10(8) M-1 s-1), leading to the formation of FNR.. No direct reduction of Fdox in the complex was observed. No change in the kinetics occurred in the presence of excess NADP+. The second-order rate constant for reduction of Fdox by 5-dRf. in the presence of a stoichiometric amount of fully ...Continue Reading

References

Aug 15, 1985·Biochemical and Biophysical Research Communications·J T Hazzard, G Tollin
Jun 1, 1968·Archives of Biochemistry and Biophysics·R D Draper, L L Ingraham
Dec 18, 1984·Biochemistry·P A KarplusJ R Herriott
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T E MeyerG Tollin
Apr 13, 1981·Biochimica Et Biophysica Acta·J M SmithD B Knaff
Jan 1, 1980·Archives of Biochemistry and Biophysics·D B KnaffR K Chain

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Citations

Sep 1, 1988·Photosynthesis Research·R PschornA Wild
Feb 1, 1994·Journal of Bioenergetics and Biomembranes·P A Karplus, C M Bruns
Oct 29, 2013·Photosynthesis Research·Taras K AntalEsa Tyystjärvi
Jan 22, 1991·Biochimica Et Biophysica Acta·D B Knaff, M Hirasawa
Aug 15, 1989·European Journal of Biochemistry·J J PueyoC Gómez-Moreno
May 18, 1994·Biochimica Et Biophysica Acta·M HirasawaD B Knaff
May 18, 2016·Journal of Photochemistry and Photobiology. B, Biology·Annamaria QuarantaPierre Sétif

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