Reduction of an eight-state mechanism of cotransport to a six-state model using a new computer program

Biophysical Journal
S FalkA Berteloot

Abstract

A computer program was developed to allow easy derivation of steady-state velocity and binding equations for multireactant mechanisms including or without rapid equilibrium segments. Its usefulness is illustrated by deriving the rate equation of the most general sequential iso ordered ter ter mechanism of cotransport in which two Na+ ions bind first to the carrier and mirror symmetry is assumed. It is demonstrated that this mechanism cannot be easily reduced to a previously proposed six-state model of Na+-D-glucose cotransport, which also includes a number of implicit assumptions. In fact, the latter model may only be valid over a restricted range of Na+ concentrations or when assuming very strong positive cooperativity for Na+ binding to the glucose symporter within a rapid equilibrium segment. We thus propose an equivalent eight-state model in which the concept of positive cooperativity is best explained within the framework of a polymeric structure of the transport protein involving a minimum number of two transport-competent and identical subunits. This model also includes an obligatory slow isomerization step between the Na+ and glucose-binding sequences, the nature of which might reflect the presence of functionally asymm...Continue Reading

References

Jan 1, 1977·Reviews of Physiology, Biochemistry and Pharmacology·R K Crane
Jan 1, 1979·Methods in Enzymology·H J Fromm
Jul 1, 1977·The Biochemical Journal·A Cornish-Bowden
Jan 1, 1992·The Journal of Membrane Biology·L ParentE M Wright
Mar 1, 1990·The Journal of Membrane Biology·G A Kimmich
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B R StevensE S Kempner
Feb 1, 1990·The American Journal of Physiology·C Smith-MaxwellG A Kimmich
Jul 1, 1990·The Journal of Membrane Biology·W WierzbickiG Roy
Jan 1, 1985·Annals of the New York Academy of Sciences·G SemenzaC M Fraser
Jan 1, 1986·The Journal of Membrane Biology·P Läuger, P Jauch
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B E Peerce, E M Wright
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K OtsuJ P Froehlich
Aug 1, 1989·The American Journal of Physiology·M E BlankD F Diedrich
Oct 1, 1988·The American Journal of Physiology·G A Kimmich, J Randles
Jan 1, 1986·The Journal of Membrane Biology·D Restrepo, G A Kimmich
May 1, 1985·The American Journal of Physiology·D Restrepo, G A Kimmich
Oct 1, 1970·Physiological Reviews·S G Schultz, P F Curran
Jan 1, 1983·The Journal of Membrane Biology·R J Turner
Dec 7, 1981·Biochimica Et Biophysica Acta·R J Turner
Mar 13, 1980·Biochimica Et Biophysica Acta·G A Kimmich, J Randles
Feb 1, 1994·The Journal of Membrane Biology·H Koepsell, J Spangenberg
Sep 5, 1993·Biochimica Et Biophysica Acta·C Gerardi-LaffinJ C Poiree
Jan 1, 1993·Annual Review of Physiology·E M Wright
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D D LooE M Wright
Apr 1, 1996·Biophysical Journal·E Bennett, G A Kimmich
Feb 1, 1996·Biophysical Journal·A SuH A Lester
Jan 15, 1997·The Journal of Membrane Biology·A HazamaE M Wright

❮ Previous
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Citations

Jul 12, 2005·The Journal of Membrane Biology·S EskandariD D F Loo
Jul 2, 2008·The Journal of Membrane Biology·Donald D F LooErnest M Wright
Oct 6, 2001·British Journal of Pharmacology·B A HirayamaE M Wright
Jul 3, 1998·The Journal of General Physiology·I ForsterH Murer
Dec 15, 2004·The Journal of General Physiology·Donald D F LooErnest M Wright
Nov 30, 2006·The Journal of General Physiology·Donald D F LooErnest M Wright
Sep 26, 2012·The Journal of General Physiology·Jean-Philippe LongpréJean-Yves Lapointe
Jul 5, 2006·Journal of Bioinformatics and Computational Biology·Tarek S NajdiEric D Mjolsness
Aug 30, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Diana Vivian, James E Polli
Oct 29, 2004·Bioinformatics·Chin-Rang YangG Wesley Hatfield
Oct 13, 2004·Journal of Molecular Biology·A ZhouK Fendler
Oct 31, 2006·Biophysical Journal·Dominique G GagnonJean-Yves Lapointe
Apr 16, 2009·Biochimica Et Biophysica Acta·Constanta Ganea, Klaus Fendler
Mar 27, 2010·Biophysical Journal·Jean-Philippe LongpréJean-Yves Lapointe
Apr 30, 2011·Physiological Reviews·Ernest M WrightBruce A Hirayama
Jan 3, 2001·American Journal of Physiology. Renal Physiology·E M Wright
Jan 20, 2005·The Journal of Biological Chemistry·Chin-Rang YangG Wesley Hatfield
Jan 1, 2006·EcoSal Plus·Kirsty A SalmonG Wesley Hatfield

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