Reduction of capillary permeability in the fructose-induced hypertensive rat

American Journal of Hypertension
M ChakirP Maheux

Abstract

Impaired insulin transcapillary transport and the subsequent decrease in insulin delivery to target organs have been suggested to play a role in insulin resistance. These defects were studied in fructose-fed rats, an animal model with insulin resistance. For this study, male Sprague-Dawley rats were fed with either a 60% fructose enriched (F) or a standard chow diet (N) for a total of 2, 4, or 8 weeks. Capillary permeability to albumin was assessed at the end of each dietary period by quantifying the extravasation of albumin-bound Evans blue (EB) dye in different organs. Unanesthetized animals were injected with Evans blue dye (20 mg/kg) in the caudal vein 10 min before being killed and EB dye was extracted by formamide from selected organs collected after exsanguination. As expected, rats had an increase in blood pressure upon feeding with fructose at 4 and 8 weeks (F, 149 +/- 3 mm Hg; N, 139 +/- 3 mm Hg; P < .05). Using this technique, we showed a 56% and a 51% reduction in capillary permeability in skeletal muscles at 4 and 8 weeks of fructose feeding, respectively (4 weeks: N, 44.5 +/- 5.0 microg/g of dry tissue; F, 19.8 +/- 4.2 microg/g of dry tissue; P < .01 and 8 weeks: N, 23.3 +/- 3.7 microg/g of dry tissue; F, 11.3 +/-...Continue Reading

Citations

Nov 15, 2008·The Journal of Pharmacology and Experimental Therapeutics·Chun Xia ZhaoDao Wen Wang
Apr 11, 2003·Journal of Pharmacological Sciences·Kazuya ShinozakiTomio Okamura
Sep 29, 2000·American Journal of Physiology. Heart and Circulatory Physiology·V C FarrS R Davis
Aug 10, 2012·Physiology·Cathryn M Kolka, Richard N Bergman

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