Reduction of cyclic and acyclic diazene derivates by Azotobacter vinelandii nitrogenase: diazirine and trans-dimethyldiazene

Biochemistry
C E McKennaM Bravo-Leerabhandh

Abstract

Nitrogenase reduces N2 to NH3, but the mechanistic details are unclear. Diazene (N2H2), a proposed 2e-/2H+ intermediate on the reduction pathway, is labile under typical enzyme assay conditions, and no firm evidence is available on whether or not it can be reduced by or inhibit nitrogenase. In this paper, we compare the interactions of Azotobacter vinelandii (Av) nitrogenase with two diazene analogues: diazirine, a photolabile diazene containing the azo (-N=N-) group in a strained, three-membered ring, and trans-dimethyldiazene, a diazene containing an unstrained trans-disubstituted N=N bond. Diazirine is reduced by nitrogenase under specific conditions to methane, methylamine, and ammonia in a ratio of ca. 1:2:4-5 with a Km value for all three products similar (0.05-0.09 mM) to that of dinitrogen (0.06-0.12 mM). The Km value of diazirine does not depend on the ratio of nitrogenase Fe protein (Av2) to nitrogenase MoFe protein (Av1) at Av2:Av1 ratios of 0.71 and 14.9. Diazirine potently and competitively inhibits acetylene reduction by Av nitrogenase with Ki = 0.03 mM and is predicted to inhibit H2 evolution completely at pressures > Km. The experimental Henry's Law constant (1.50 M/atm) determined for trans-dimethyldiazene in H...Continue Reading

References

Feb 10, 1988·Biochimica Et Biophysica Acta·R W Miller, R R Eady

❮ Previous
Next ❯

Citations

Mar 24, 2011·Analytical Chemistry·Chanelle C Jumper, David C Schriemer
Jul 10, 2013·Journal of the American Chemical Society·Feng NiCharles E McKenna
May 1, 2008·Journal of the American Chemical Society·Ying YuPatrick L Holland
May 12, 2006·Dalton Transactions : an International Journal of Inorganic Chemistry·Brett M BarneyLance C Seefeldt
Jan 29, 2014·Chemical Reviews·Brian M HoffmanLance C Seefeldt
Apr 20, 2013·Biochimica Et Biophysica Acta·Lance C SeefeldtDennis R Dean
Oct 17, 2013·Chemical Communications : Chem Comm·Ian Dance
Mar 17, 2020·Chemical Reviews·Lance C SeefeldtBrian M Hoffman
Apr 3, 2020·Chemical Reviews·Casey Van StappenSerena DeBeer
Jun 24, 2008·The Journal of Organic Chemistry·Carina Gaviglio, Fabio Doctorovich
Jan 17, 2019·Inorganic Chemistry·Albert Th Thorhallsson, Ragnar Bjornsson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.