PMID: 11342267May 9, 2001Paper

Reduction of dehydroascorbic acid by homocysteine

Biochimica Et Biophysica Acta
J B Park

Abstract

To determine the reductive process of extracellular dehydroascorbic acid (DHA), molecules (homocysteine, homocysteine thiolactone, methionine, cysteine, and homoserine) were tested to identify those with the potential to reduce DHA to ascorbic acid (AA). Homocysteine (Hcy) was the most potent of the molecules tested. The efficacy of Hcy was compared with that of other molecules able to reduce DHA (reduced glutathione (GSH) and cysteine (Cy)). Although all three molecules were able to reduce DHA, GSH and Cy were not to reduce DHA to AA at concentrations lower than 100 micromol/l, and only less than 5% DHA was reduced to AA at concentrations of 200-300 micromol/l. In contrast, Hcy reduced DHA to AA stoichiometrically at concentrations as low as 10 micromol/l. In Jurkat and U937 cells, the increasing concentrations of extracellular Hcy suppressed intracellular dehydroascorbic acid uptake, indicating that extracellular reduction of DHA by Hcy leads to decreasing extracellular DHA available for its intracellular uptake. Simultaneous oxidation and reduction of Hcy and DHA were accelerated extracellularly in the presence of quercetin, an inhibitor of DHA uptake, suggesting that extracellular ascorbic acid concentration increased via b...Continue Reading

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Citations

Mar 26, 2003·Free Radical Biology & Medicine·Rushdi H AlulSean M Lynch
Mar 18, 2004·Journal of the American Chemical Society·Weihua WangRobert M Strongin
Feb 27, 2018·Amino Acids·Kamila BorowczykHieronim Jakubowski
Mar 3, 2020·Rapid Communications in Mass Spectrometry : RCM·Grace Kouakou AhuieKlaus Klarskov
Jul 15, 2017·Antioxidants·Kimberly SanfordRamesh Natarajan
May 18, 2017·Stem Cells International·Cristina D'AnielloGabriella Minchiotti
May 16, 2014·Chemical Communications : Chem Comm·Cyril BachmannRoger Alberto
Apr 4, 2021·Antioxidants·Borut PoljsakIrina Milisav
Apr 4, 2006·Archives of Biochemistry and Biophysics·Mikhail LinetskyOgnyan K Argirov
Jun 23, 2011·Journal of the American Chemical Society·Sarah E BohndiekKevin M Brindle
May 25, 2010·Archives of Biochemistry and Biophysics·Alessandro CortiAlfonso Pompella

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