Reduction of Dicer impairs Schwann cell differentiation and myelination.

Journal of Neuroscience Research
Jonathan D VerrierLucia Notterpek

Abstract

The process of Schwann cell myelination requires precisely coordinated gene expression. At the onset of myelination, there is an increase in the expression of differentiation-promoting transcription factors that regulate key Schwann cell genes. Further control of myelin gene expression occurs at the posttranscriptional level and, in part, is mediated by RNA binding proteins and micro-RNAs (miRNAs). miRNAs are small, endogenously derived RNA molecules that repress gene expression by specifically binding to their mRNA targets. In the experiments described here, we tested whether miRNAs were essential in controlling myelination by reducing the levels of Dicer, an essential endoribonuclease in miRNA biogenesis. We decreased the expression of Dicer by about 60% within Schwann cells using a lentiviral vector expressing an shRNA against Dicer. The reduced levels of Dicer led to a decrease in the steady-state expression of selected miRNAs and of the transcription factors Oct6 and Egr2/Krox20, both of which are critical for Schwann cells differentiation and myelination. In contrast, the levels of c-jun and Sox2 were up-regulated by the reduction in Dicer and were associated with an increase in Schwann cell proliferation. In dorsal root ...Continue Reading

References

Oct 1, 1992·Brain Pathology·J R Lupski, C A Garcia
Oct 23, 1997·Current Opinion in Neurology·S S Scherer
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 20, 2002·Neurobiology of Disease·Mary C RyanLucia Notterpek
Nov 2, 2002·The EMBO Journal·Patrick ProvostOlof Rådmark
Oct 7, 2003·Nature Genetics·Emily BernsteinGregory J Hannon
Feb 6, 2004·The Journal of Cell Biology·David B ParkinsonKristjan R Jessen
Sep 17, 2004·Nature·Victor Ambros
Feb 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nam LeJeffrey Milbrandt
Feb 17, 2005·Genes & Development·Chryssa KanellopoulouKlaus Rajewsky
Jul 13, 2005·The Journal of Experimental Medicine·Stefan A MuljoKlaus Rajewsky
Aug 16, 2005·Current Opinion in Genetics & Development·Eric A Miska
Feb 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kelley S HarrisXin Sun
Mar 3, 2006·Genes & Development·Marco Antonio Valencia-SanchezRoy Parker
Aug 23, 2006·Journal of Neurochemistry·Sung-Wook JangJohn Svaren
Apr 3, 2007·Nature Genetics·Madhu S KumarTyler Jacks
Sep 6, 2007·Cell Cycle·Michael CarletonPeter S Linsley
Sep 7, 2007·Diabetes·Francis C LynnMichael S German
Sep 18, 2007·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Rahima A BhanjiMarvin J Fritzler
Oct 16, 2007·Developmental Biology·Jason R O'RourkeBrian D Harfe
Feb 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jian-Fu ChenDa-Zhi Wang
Apr 25, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tigwa H DavisErik M Ullian
May 21, 2008·The Journal of Cell Biology·David B ParkinsonKristján R Jessen
Jul 2, 2008·Biochimica Et Biophysica Acta·N Ruth ZearfossSean P Ryder
Jul 29, 2008·Neurobiology of Disease·Sunitha RangarajuLucia Notterpek
Aug 20, 2008·Molecular and Cellular Biology·Deborah A Greer CardTrevor K Archer
Nov 4, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Satoshi AsadaHidemasa Oh
Nov 7, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Pierre LauLynn D Hudson
Jan 27, 2009·Glia·Jonathan D VerrierLucia Notterpek
Mar 5, 2009·Disease Models & Mechanisms·Shu-Ting Lin, Ying-Hui Fu
Apr 10, 2009·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Steve VucicDavid R Cornblath
Apr 28, 2009·Cell Biology International·Nicoletta PotenzaAniello Russo
Jun 3, 2009·The Journal of Biological Chemistry·Sung-Wook Jang, John Svaren
Jun 16, 2009·Nature Neuroscience·Ashwin WoodhooKristján R Jessen

❮ Previous
Next ❯

Citations

Nov 9, 2011·Molecular and Cellular Biology·Nolan G GokeyJohn Svaren
Feb 25, 2011·Developmental Neuroscience·Jason C Dugas, Lucia Notterpek
May 4, 2011·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Xuelian HeQ Richard Lu
Dec 2, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Andreu ViaderJeffrey Milbrandt
Jul 1, 2014·Neurochemistry International·John Svaren
Oct 4, 2015·Progress in Neurobiology·Bin YuXiaosong Gu
Sep 1, 2015·PloS One·Christina MüllerRobin White
Nov 25, 2017·Neural Regeneration Research·Sheng YiSong-Lin Zhou
Jan 10, 2014·Developmental Neurobiology·Carolina UggentiAda Maria Tata
Jul 6, 2014·Viruses·Charlotte LemppFrauke Seehusen
Sep 14, 2014·Human Molecular Genetics·Darin J FalkBarry J Byrne
Aug 16, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Susu MaoBin Yu
Jul 7, 2020·Experimental Neurobiology·Rajarathinam ThenmozhiYoung Bin Hong
May 2, 2017·BioMed Research International·Xian-Hu ZhouShi-Qing Feng
Jun 2, 2018·BioMed Research International·Eun Jung Sohn, Hwan Tae Park
Jul 3, 2021·International Journal of Molecular Sciences·Marina StavrouKleopas A Kleopa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.