PMID: 8967465May 1, 1996Paper

Reduction of dietary obesity in aP2-Ucp transgenic mice: mechanism and adipose tissue morphology

The American Journal of Physiology
Jan KopeckyP Kolárová

Abstract

C57BL6/J mice with the expression of the mitochondrial uncoupling protein (UCP) gene from the fat-specific aP2 gene promoter were used to study the mechanism by which the aP2-Ucp transgene affects adiposity and reduces high-fat diet induced obesity. In the transgenic mice, UCP synthesized in white fat was inserted into mitochondria, and oxygen uptake by epididymal fat fragments indicated UCP-induced thermogenesis. The respirometry data, UCP content, cytochrome oxidase activity, and tissue morphology suggested functional involution of brown fat. Despite 25- to 50-fold lower mitochondrial cytochrome oxidase activity in white than in brown fat cells, total oxidative capacity in white and brown adipose tissue is comparable. Appearance of novel small cells in the gonadal fat of the transgenic mice was associated with a higher DNA content than that of the nontransgenic mice. The results prove a potential of transgenically altered mitochondria in white fat to modulate adiposity and energy expenditure and suggest the existence of a yet unidentified site-specific link between energy metabolism in adipocytes and cellularity.

Citations

Sep 10, 2003·Journal of Applied Physiology·Yoriko MasudaTohru Fushiki
Aug 17, 2014·Experimental Physiology·Sihem Boudina, Timothy E Graham
Nov 25, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nathan C WinnJaume Padilla
Aug 10, 2018·The Journal of Endocrinology·Stephanie L ClookeyVictoria Vieira-Potter
Jun 30, 2016·American Journal of Physiology. Endocrinology and Metabolism·Christopher J Madden, Shaun F Morrison
Jun 29, 2017·American Journal of Physiology. Endocrinology and Metabolism·Nathan C WinnJaume Padilla
Jan 28, 2004·Annual Review of Pharmacology and Toxicology·Jacques RobidouxSheila Collins
Apr 15, 2008·Annual Review of Nutrition·Mary-Ellen HarperMartin D Brand
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
May 22, 2008·American Journal of Physiology. Endocrinology and Metabolism·Vladimir KusJan Kopecky
Dec 1, 2020·American Journal of Physiology. Endocrinology and Metabolism·Hui WangMartin Klingenspor
Jul 2, 2021·Biological & Pharmaceutical Bulletin·Yasuhiro NakamuraKen Karasawa

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