Reduction of Escherichia coli O157:H7 in Fresh Spinach, using lactic acid bacteria and chlorine as a multihurdle intervention

Journal of Food Protection
S E Gragg, M M Brashears

Abstract

A 12-day shelf life study was conducted at 7 degrees C to determine whether Escherichia coli O157:H7 on spinach can be controlled effectively by selected strains of lactic acid bacteria (LAB) alone or in combination with chlorine as a multihurdle intervention. The multihurdle intervention consisted of both LAB and chlorine and was applied to spinach as a rinse and evaluated in comparison to LAB alone and chlorine and water rinses. Reductions achieved by all treatments also were compared with those observed for an inoculated control. The spinach was inoculated by submersion in a solution containing an E. coli O157:H7 cocktail at 1.0 x 10(6) CFU/ml. LAB were applied postharvest at a concentration of 2.0 x 10(8) CFU/ml, and 200 ppm of chlorine was used for the chlorine rinse. All spinach samples were packaged in commercial packaging, held in a retail display case, and tested for E. coli O157:H7 on days 0, 1, 3, 6, 9, and 12 using the Neo-Grid filtration system and CHROMagar. Survival of LAB throughout the shelf life also was determined. Significant reductions in pathogen populations were achieved by water (P = 0.0008), LAB (P < 0.0001), chlorine (P < 0.0001), and multihurdle (P < 0.0001) treatments when compared with controls. The...Continue Reading

References

Jun 1, 1994·The Pediatric Infectious Disease Journal·L K PickeringF B Stapleton
Feb 19, 2002·Journal of Food Protection·A Amézquita, M M Brashears
Dec 24, 2002·Journal of Food Protection·Karen M Schuenzel, Mark A Harrison
Oct 24, 2003·Journal of Food Protection·Keith WarrinerWilliam M Waites
May 5, 2005·Applied and Environmental Microbiology·Gro S JohannessenLiv Marit Rørvik
Dec 1, 2006·Journal of Food Protection·Tinna A MananiSisai Mpuchane
Jan 12, 2007·Canadian Journal of Microbiology·M Kostrzynska, A Bachand
Sep 7, 2007·Journal of Food Protection·Pascal DelaquisLaura-Dorina Dinu
Jan 29, 2008·Recent Patents on Anti-infective Drug Discovery·Yolanda SanzEster Sánchez

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