Reduction of invasive bacteria in ethanol fermentations using bacteriophages

Biotechnology and Bioengineering
Thomas O Worley-MorseClaudia K Gunsch

Abstract

Invasive Lactobacillus bacteria inhibit ethanol fermentations and reduce final product yields. Due to the emergence of antibiotic resistant strains of Lactobacillus spp., alternative disinfection strategies are needed for ethanol fermentations. The feasibility of using the bacteriophage (phage) 8014-B2 to control Lactobacillus plantarum in ethanol fermentations by Saccharomyces cerevisiae was investigated. In 48 h media-based shake flask fermentations, phages achieved greater than 3-log inactivation of L. plantarum, protected final ethanol yields, and maintained yeast viability. The phage-based bacterial disinfection rates depended on both the initial phage and bacterial concentrations. Furthermore, a simple set of kinetic equations was used to model the yeast, bacteria, phage, reducing sugars, and ethanol concentrations over the course of 48 h, and the various kinetic parameters were determined. Taken together, these results demonstrate the applicability of phages to reduce L. plantarum contamination and to protect final product yields in media-based fermentations.

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Citations

Sep 4, 2015·Virology·Zachary J Storms, Dominic Sauvageau
Jun 3, 2017·Critical Reviews in Biotechnology·Ramon Peres Brexó, Anderson de Souza Sant'Ana
Nov 11, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Luciano Lopes QueirozGustavo Augusto Lacorte

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