Reduction of lesion growth rate of late blight plant disease in transgenic potato expressing harpin protein

Science in China. Series C, Life Sciences
R Li, Y Fan

Abstract

Using harpin protein gene from apple fire blight pathogen Erwinia amylovora and potato prp1-1 promoter as main DNA elements, the feasibility of using pathogen infection-induced hypersensitive response was explored as a new strategy of engineering fungal disease resistance. Three plant transformation vectors were constructed and 68 transgenic potato plants were produced through Agrobacterium mediated transformation method. Southern, Northern and Western blot analysis demonstrated the insertion, transcription and protein expression of harpin protein gene in transgenic plants. Disease resistance test using a complex race of Phytophthora infestans as challenging pathogen showed that both constitutive and pathogen infection-induced expression of harpin protein gene in transgenic potato reduced the lesion growth rate of fungus. Among plants where harpin protein gene expression was induced only by fungus infection, two plants were found to be highly resistant to P.infestans infection. Fungal hyphae were not produced on total of 30 inoculated leaves from the two resistant plants and necrotic lesion was limited to inoculation area. The results highlighted that engineered hypersensitive response in plants was a very promising approach to...Continue Reading

References

Jul 1, 1992·Molecular Plant-microbe Interactions : MPMI·U M Pfitzner, A J Pfitzner
Jul 1, 1995·Transgenic Research·F A van EngelenW J Stiekema
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·K E Hammond-KosackJ D Jones
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Jean T. Greenberg

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Citations

Jan 16, 2014·Journal of Food Science and Technology·Himmet TezcanBulent Akbudak
Aug 2, 2008·International Journal of Food Sciences and Nutrition·Bulent AkbudakAtilla Eris

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