Reduction of lipopolysaccharide-induced interleukin-6 production by the kappa opioid U50,488 in a mouse monocyte-like cell line

International Immunopharmacology
Amy L Parkhill, J M Bidlack

Abstract

Several studies demonstrate that opioids modulate the immune response via opioid receptors expressed directly on the immune cells themselves. Recently, it has been suggested that the kappa opioid system has a modulatory role in various inflammatory diseases including rheumatoid arthritis. This modulation may occur via changes in cytokine secretion by monocyte-derived cells. To further study this opioid-immune relationship, we stimulated P388D1 cells, a mouse monocyte-like cell line, with lipopolysaccharide (LPS) in the presence or absence of the kappa opioid-selective ligand, U50,488. Pretreatment with U50,488 significantly reduced LPS-stimulated interleukin-6 (IL-6) production as measured by ELISA. This effect was mediated by the kappa opioid receptor, because nor-binaltorphimine (nor-BNI), a kappa-selective antagonist, blocked this inhibition. It is likely that this reduction of IL-6 protein by U50,488 treatment is attributed to decreases in IL-6 mRNA. RT-PCR experiments demonstrated that U50,488 treatment significantly reduced the LPS-mediated increase in IL-6 mRNA and that this effect was also blocked by nor-BNI. Understanding the mechanism behind the reduction of proinflammatory cytokine production by opioids may lead to t...Continue Reading

References

Jan 1, 1989·Cellular Immunology·M J MelnicoffP S Morahan
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·J D BretzR C Schwartz
Jan 1, 1996·Journal of Cardiovascular Pharmacology·J H Levy
May 8, 1998·Biochemical and Biophysical Research Communications·S RoyR A Barke
Jun 4, 1998·Journal of Neuroimmunology·B M SharpJ M Bidlack
Dec 10, 1998·Journal of Neuroimmunology·C AliceaT J Rogers
Sep 6, 2000·Clinical and Diagnostic Laboratory Immunology·J M Bidlack
Mar 14, 2001·Drug and Alcohol Dependence·C C ChaoP K Peterson
Sep 11, 2002·Cytokine & Growth Factor Reviews·Katsuhiko Ishihara, Toshio Hirano
Jun 10, 2003·Annals of the New York Academy of Sciences·Paola Sacerdote
Jul 15, 2004·Current Opinion in Pharmacology·Norihiro Nishimoto, Tadamitsu Kishimoto
Apr 6, 2005·Clinical and Experimental Immunology·F Goldblatt, D A Isenberg

❮ Previous
Next ❯

Citations

Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jean M BidlackChristopher M Tipton
Jun 24, 2014·Biochemistry. Biokhimii︠a︡·S V Gein
Oct 11, 2012·Journal of Perinatology : Official Journal of the California Perinatal Association·R Chavez-ValdezE B Gauda
Sep 2, 2008·Developmental and Comparative Immunology·Magdalena ChadzinskaB M Lidy Verburg-van Kemenade
Mar 20, 2012·Journal of Neuroimmunology·Jelka GabrilovacAnamaria Brozovic
Dec 15, 2010·Archives of Oral Biology·Jasílio Vilela BastosKátia Lucy de Melo Maltos
Nov 23, 2017·Trends in Molecular Medicine·Luis UlloaRafael Torres-Rosas
Oct 23, 2020·Archives of Oral Biology·Marcelo Queiroz D'ÂngeloRodrigo Villamarim Soares

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.