Reduction of methemoglobin via electron transfer from photoreduced flavin: restoration of O2-binding of concentrated hemoglobin solution coencapsulated in phospholipid vesicles

Bioconjugate Chemistry
Hiromi SakaiEishun Tsuchida

Abstract

Ferric methemoglobin is reduced to its ferrous form by photoirradiation either by direct photoexcitation of the heme portion to induce electron transfer from the surrounding media (Sakai at al. (2000) Biochemistry 39, 14595-14602) or by an indirect electron transfer from a photochemically reduced electron mediator such as flavin. In this research, we studied the mechanism and optimal condition that facilitates photoreduction of flavin mononucleotide (FMN) to FMNH(2) by irradiation of visible light, and the succeeding reduction of concentrated metHb in phospholipid vesicles to restore its O(2) binding ability. Visible light irradiation (435 nm) of a metHb solution containing FMN and an electron donor such as EDTA showed a significantly fast reduction to ferrous Hb with a quantum yield (Phi) of 0.17, that is higher than the method of direct photoexcitation of heme (Phi = 0.006). Electron transfer from a donor molecule to metHb via FMN was completed within 30 ns. Native-PAGE and IEF electrophoresis indicated no chemical modification of the surface of the reduced Hb. Coencapsulation of concentrated Hb solution (35 g/dL) and the FMN/EDTA system in vesicles covered with a phospholipid bilayer membrane (Hb-vesicles, HbV, diameter: 250...Continue Reading

Citations

May 14, 2014·Annual Review of Biomedical Engineering·Andre F Palmer, Marcos Intaglietta
Sep 1, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Yasuyuki Yamada, Shin Aoki
Nov 17, 2009·Methods in Enzymology·Hiromi SakaiEishun Tsuchida
Mar 28, 2009·International Journal of Molecular Sciences·Jing ZhaoGenxi Li
Jun 17, 2006·Pharmaceutical Research·Patrick Couvreur, Christine Vauthier
Feb 12, 2011·Macromolecular Bioscience·Taihang LiYubin Huang
Jul 30, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Hiromi Sakai, Cassandra Leong
Jan 12, 2021·International Journal of Biological Macromolecules·Manish ShandilyaSuman Kundu
Feb 12, 2009·Bioconjugate Chemistry·Eishun TsuchidaKoichi Kobayashi
Dec 19, 2007·Journal of Photochemistry and Photobiology. B, Biology·Xiaoqiang LiangGenxi Li

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