Reduction of nitrite to nitric oxide during ischemia protects against myocardial ischemia-reperfusion damage

Proceedings of the National Academy of Sciences of the United States of America
Andrew WebbAmrita Ahluwalia

Abstract

Nitric oxide (NO.) is thought to protect against the damaging effects of myocardial ischemia-reperfusion injury, whereas xanthine oxidoreductase (XOR) normally causes damage through the generation of reactive oxygen species. In the heart, inorganic nitrite (NO(2)(-)) has the potential to act as an endogenous store of NO., liberated specifically during ischemia. Using a detection method that we developed, we report that under ischemic conditions both rat and human homogenized myocardium and the isolated perfused rat heart (Langendorff preparation) generate NO. from NO(2)(-) in a reaction that depends on XOR activity. Functional studies of rat hearts in the Langendorff apparatus showed that nitrite (10 and 100 microM) reduced infarct size from 47.3 +/- 2.8% (mean percent of control +/- SEM) to 17.9 +/- 4.2% and 17.4 +/- 1.0%, respectively (P < 0.001), and was associated with comparable improvements in recovery of left ventricular function. This protective effect was completely blocked by the NO. scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazole-1-oxyl 3-oxide (carboxy-PTIO). In summary, the generation of NO. from NO(2)(-), by XOR, protects the myocardium from ischemia-reperfusion injury. Hence, if XOR is presented with N...Continue Reading

References

Nov 1, 1990·British Journal of Pharmacology·D D ReesS Moncada
Aug 1, 1995·Nature Medicine·J L ZweierP Kuppusamy
Apr 7, 1995·The Journal of Biological Chemistry·K Okamoto, T Nishino
Apr 7, 1994·Nature·N BenjaminH McKenzie
Apr 7, 1994·Nature·V E Buckwold
Aug 7, 1993·Biochimica Et Biophysica Acta·Y MoriwakiK Higashino
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·X L MaM Feelisch
Feb 1, 2000·Journal of Molecular and Cellular Cardiology·M S SumerayD M Yellon
Mar 14, 2000·The Journal of Biological Chemistry·B L GodberR Harrison
Jun 20, 2000·FEBS Letters·B L GodberR Harrison
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·M T GladwinR O Cannon
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·B BeltránS Moncada
Mar 30, 2001·The Journal of Biological Chemistry·U S KayyaliP M Hassoun
Apr 11, 2001·Trends in Biochemical Sciences·M Brunori
May 15, 2001·Acta Physiologica Scandinavica·A ModinJ O Lundberg
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·T LauerM Kelm
May 8, 2002·Nature Reviews. Molecular Cell Biology·Salvador Moncada, Jorge D Erusalimsky
Sep 5, 2002·Free Radical Biology & Medicine·Roger Harrison
Dec 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Juan RodriguezMartin Feelisch
Dec 31, 2002·Cardiovascular Research·Sharmila ChauhanAmrita Ahluwalia
Nov 5, 2003·Nature Medicine·Kenyatta CosbyMark T Gladwin
Dec 25, 2003·The Journal of Physiology·Cristine E Berry, Joshua M Hare
Jan 1, 2004·The Journal of Clinical Investigation·HåKan Björne HJon O Lundberg
Feb 10, 2004·Biochemical and Biophysical Research Communications·Mohammad Saleem, Hiroshi Ohshima
Feb 10, 2004·The Journal of Biological Chemistry·Haitao LiJay L Zweier

❮ Previous
Next ❯

Citations

May 15, 2007·Intensive Care Medicine·Johanna AlbertWaldemar Goździk
Nov 27, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Prabal K Chatterjee
Jun 1, 2011·European Journal of Nutrition·Ajay Machha, Alan N Schechter
Feb 27, 2009·Basic Research in Cardiology·Sruti Shiva, Mark T Gladwin
Jan 21, 2010·Plant Foods for Human Nutrition·Verónica PiñeiroGermán Chamorro-Cevallos
Sep 4, 2009·Archives of Pharmacal Research·Jon O Lundberg, Eddie Weitzberg
Sep 4, 2009·Archives of Pharmacal Research·David J Lefer
Jun 15, 2007·Cardiovascular Research·Cameron DezfulianMark T Gladwin
Jun 14, 2005·Pharmacology & Therapeutics·R SchulzJ-L Balligand
Dec 15, 2006·Journal of the American Chemical Society·Harinath ChakrapaniS Bruce King
Jun 3, 2008·Nature Chemical Biology·Emmelie A JanssonJon O Lundberg
May 28, 2013·Nature Medicine·Edward T ChouchaniMichael P Murphy
Jan 3, 2008·Nature Reviews. Drug Discovery·Jon O LundbergMark T Gladwin
Jun 10, 2010·The Pharmacogenomics Journal·I F MetzgerJ E Tanus-Santos
Jun 15, 2007·Dalton Transactions : an International Journal of Inorganic Chemistry·Nalinava Sen GuptaGoutam Kumar Lahiri
Dec 22, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·Elena A VlasovaRudi van Eldik
Aug 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·John W ElrodDavid J Lefer
Jul 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ulrike B Hendgen-CottaTienush Rassaf
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mattias CarlströmJon O Lundberg
Aug 2, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jon O Lundberg
Aug 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jiangning YangJohn Pernow
Aug 8, 2007·The Journal of Experimental Medicine·Sruti ShivaMark T Gladwin
Sep 22, 2006·Antioxidants & Redox Signaling·Junhui SunElizabeth Murphy
Dec 7, 2007·Antioxidants & Redox Signaling·Lindsay S Burwell, Paul S Brookes
Nov 19, 2008·Antioxidants & Redox Signaling·M Carmen Martínez, Ramaroson Andriantsitohaina
Feb 11, 2009·Antioxidants & Redox Signaling·Mohammad N Jameel, Jianyi Zhang
Dec 17, 2009·Antioxidants & Redox Signaling·Amadou K S CamaraDavid F Stowe
Jun 4, 2010·Antioxidants & Redox Signaling·Bruno TotaMaria Carmela Cerra
Feb 7, 2012·Antioxidants & Redox Signaling·Nicoletta CastiglioneFrancesca Cutruzzolà
Nov 22, 2011·Antioxidants & Redox Signaling·Erin CurtisSruti Shiva
Mar 3, 2009·Cardiovascular Research·John W Calvert, David J Lefer
Jul 28, 2010·Cardiovascular Research·Qian-Chen YongJin-Song Bian
Oct 5, 2010·Cardiovascular Research·Julian O M OrmerodMichael P Frenneaux
Sep 30, 2010·Cardiovascular Research·John W Calvert
Oct 5, 2010·Cardiovascular Research·Mark GilchristNigel Benjamin
Oct 19, 2010·Cardiovascular Research·Rakesh P PatelDaniel B Kim-Shapiro
Oct 13, 2010·Cardiovascular Research·Jon O LundbergEddie Weitzberg
Jul 19, 2013·European Heart Journal·Thomas MünzelTommaso Gori
Mar 26, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Heather B LeavesleyGary E Isom

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