Reduction of Oxidative Damage and Inflammatory Response in the Diaphragm Muscle of mdx Mice Using Iron Chelator Deferoxamine

Biological Trace Element Research
Luis Henrique Rapucci MoraesElaine Minatel

Abstract

Oxidative stress and inflammatory processes strongly contribute to pathogenesis in Duchenne muscular dystrophy (DMD). Based on evidence that excess iron may increase oxidative stress and contribute to the inflammatory response, we investigated whether deferoxamine (DFX), a potent iron chelating agent, reduces oxidative stress and inflammation in the diaphragm (DIA) muscle of mdx mice (an experimental model of DMD). Fourteen-day-old mdx mice received daily intraperitoneal injections of DFX at a dose of 150 mg/kg body weight, diluted in saline, for 14 days. C57BL/10 and control mdx mice received daily intraperitoneal injections of saline only, for 14 days. Grip strength was evaluated as a functional measure, and blood samples were collected for biochemical assessment of muscle fiber degeneration. In addition, the DIA muscle was removed and processed for histopathology and Western blotting analysis. In mdx mice, DFX reduced muscle damage and loss of muscle strength. DFX treatment also resulted in a significant reduction of dystrophic inflammatory processes, as indicated by decreases in the inflammatory area and in NF-κB levels. DFX significantly decreased oxidative damage, as shown by lower levels of 4-hydroxynonenal and a reducti...Continue Reading

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Citations

Dec 8, 2015·Clinical and Experimental Pharmacology & Physiology·Túlio de Almeida HermesElaine Minatel
Jan 23, 2016·Oxidative Medicine and Cellular Longevity·Min Hee ChoiReshma Taneja
Aug 18, 2016·Seminars in Cell & Developmental Biology·Maryline Moulin, Ana Ferreiro
Aug 9, 2017·PloS One·Rafael Dias MâncioElaine Minatel
Apr 23, 2019·PloS One·Túlio de Almeida HermesElaine Minatel
Jul 5, 2018·Journal of Cancer Research and Clinical Oncology·Silvia CodenottiAlessandro Fanzani
Aug 23, 2020·International Journal of Molecular Sciences·Alexander Mensch, Stephan Zierz

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