Reduction of plastid-localized carbonic anhydrase activity results in reduced Arabidopsis seedling survivorship.

Plant Physiology
Fernando J FerreiraJ R Coleman

Abstract

Carbonic anhydrase (CA; EC 4.2.1.1) catalyzes the interconversion of CO2 and HCO3(-) and is a major protein constituent of the C3 higher plant chloroplast where it is presumed to play a role in photosynthetic carbon assimilation. In this study, we have used both RNA antisense and gene knockout lines to specifically reduce the activity of the chloroplast betaCA1 polypeptide (At3g01500) in the model plant Arabidopsis (Arabidopsis thaliana). Although able to germinate, seedling establishment of transgenic plants is significantly reduced relative to wild-type plants when grown at ambient levels of CO2. Growth at elevated (1,500 microL L(-1)) CO2 or on plates supplemented with sucrose restores seedling establishment rates to wild-type levels. Seed from wild-type and transgenic plants exhibited no significant differences in seed protein, lipid content, or reserve mobilization during seedling growth. betaCA1-deficient seedlings do, however, exhibit reduced capacity for light-dependent 14CO2 assimilation prior to the development of true leaves. The small number of surviving seedlings able to grow and develop are phenotypically similar to wild-type plants, even when subsequently grown at subambient levels of CO2. Microarray analysis of ...Continue Reading

References

Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·H X ZhangE Blumwald
Feb 14, 2002·Plant Physiology·Yih-Kuang Lu, Alan J Stemler
Jan 10, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Amy M RocklinJohn D Lipscomb
Dec 18, 2004·Plant Molecular Biology·Gustavo ParisiEduardo Zabaleta
Mar 25, 2005·Biochemical Society Transactions·S PenfieldI A Graham
Oct 15, 2005·Plant & Cell Physiology·Yih-Kuang LuAlan J Stemler
Jan 13, 2006·Plant Physiology·Toshitsugu NakanoHideaki Shinshi
Apr 21, 2006·The Plant Journal : for Cell and Molecular Biology·Taishi UmezawaKazuo Shinozaki
Aug 1, 1990·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B LemieuxJ Browse

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Citations

Feb 16, 2011·Journal of Experimental Botany·Sylvain AubryJulian M Hibberd
Mar 17, 2011·Journal of Experimental Botany·Martha Ludwig
Dec 2, 2011·Journal of the Royal Society, Interface·Georg BaslerZoran Nikoloski
Jul 13, 2013·International Journal of Biological Sciences·Chunming WangJianmin Wan
Jan 5, 2014·Planta·Werner E G MüllerXiaohong Wang
Mar 13, 2014·Journal of Experimental Botany·John A Raven
Jun 3, 2011·Plant, Cell & Environment·Martha Ludwig
Mar 27, 2016·Current Opinion in Plant Biology·Martha Ludwig
Oct 17, 2009·Microbiology·Skander Elleuche, Stefanie Pöggeler
Nov 5, 2015·Biochemistry. Biokhimii︠a︡·N N RudenkoB N Ivanov
Dec 3, 2014·Plant Science : an International Journal of Experimental Plant Biology·Dhirendra Kumar
Mar 15, 2015·Frontiers in Plant Science·Abir U Igamberdiev
Feb 13, 2016·The Plant Journal : for Cell and Molecular Biology·Maureen R HansonMartin A J Parry
Feb 1, 2017·The Plant Journal : for Cell and Molecular Biology·Taiyu ChenYongjun Lin
Jan 28, 2017·Current Opinion in Biotechnology·Tobias W Giessen, Pamela A Silver
Jul 29, 2017·PloS One·Laura Medina-PuchePablo Tornero
Jul 20, 2019·Plants·Cátia BritoCarlos M Correia
Dec 10, 2019·The Plant Journal : for Cell and Molecular Biology·Mina MomayyeziRobert D Guy
May 13, 2018·International Journal of Molecular Sciences·Charlotte PoschenriederJuan Barceló
Apr 19, 2019·Metabolites·Lyudmila IgnatovaBoris Ivanov
Aug 6, 2020·Biomolecules·Anna DreyerKarl-Josef Dietz
Sep 21, 2016·Molecular Plant·Robert J DiMarioJames V Moroney
Mar 28, 2021·Science Advances·Youngwoo Lee, Daniel B Szymanski
Aug 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kevin M HinesMaureen R Hanson

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