Reduction of protein kinase C α (PKC-α) promote apoptosis via down-regulation of Dicer in bladder cancer

Journal of Cellular and Molecular Medicine
Zhenming JiangXi Chen

Abstract

In clinic, we examined the expression of protein kinase C (PKC)-α and Dicer in the samples of bladder cancer patients, and found that the two proteins have a line correlation. Our study confirmed this correlation existing by clearing the decreasing expression of Dicer after the PKC-α knockdown. Treatment of bladder cancer cell lines (T24, 5637) with the PKC-α or Dicer knockdown and the PKC inhibitors (Calphostin C and Gö 6976) can promote the apoptosis. Inhibition of PKC can increase the activities of caspase-3 and PARP, however, decrease the expression of Dicer. And knockdown of the PKC-α or Dicer can also activate the caspase-3 or the PARP. Considering the reduction of PKC-α can induce the Dicer down-regulation, we make the conclusion that the reduction of PKC-α can promote the apoptosis via the down-regulation of Dicer in bladder cancer.

References

May 1, 1973·International Journal of Cancer. Journal International Du Cancer·J BubeníkJ Donner
Oct 1, 1996·Molecular and Cellular Biochemistry·H J Brömme, J Holtz
Jun 26, 1998·The Biochemical Journal·H Mellor, P J Parker
Jul 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Brian D HarfeClifford J Tabin
Mar 3, 2007·Cancer Research·Simion ChioseaSanja Dacic
Feb 22, 2008·The Biochemical Journal·Alexey A Matskevich, Karin Moelling
Aug 14, 2008·Carcinogenesis·Shogo TokumaruTakashi Takahashi
Jul 24, 2012·Breast Cancer Research and Treatment·Sarkawt M KhoshnawAndrew R Green
Jul 13, 2013·The Journal of Biological Chemistry·Ganeshkumar RajendranSoumen Paul
Nov 14, 2013·The Journal of Biological Chemistry·Patricia A J MullerKaren H Vousden
Dec 11, 2013·The Journal of Biological Chemistry·Steven G ChaulkXaralabos Varelas

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
PCR
FACS

Software Mentioned

- Quest
SPSS
Cell

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis