PMID: 2500063Jul 1, 1989Paper

Reduction of protein mixed disulfides (dethiolation) by Escherichia coli thioredoxin: a study with glycogen phosphorylase b and creatine kinase

Archives of Biochemistry and Biophysics
E M Park, J A Thomas

Abstract

The role of thioredoxin in the reduction of protein mixed disulfides (dethiolation) was studied by electrofocusing methodology with glycogen phosphorylase b and creatine kinase as substrates for the reaction. Glycogen phosphorylase b was effectively dethiolated by Escherichia coli thioredoxin with dithiothreitol as the reductant, while creatine kinase could not be dethiolated by this mechanism. The rate of dethiolation of phosphorylase b was dependent on the concentration of thioredoxin up to a maximum at 20 microM when the concentration of phosphorylase b was 4 microM in monomer. Rat heart contained a thioredoxin reductase activity that could use added E. coli thioredoxin to dethiolate phosphorylase b and the same concentration of thioredoxin as above was required. This activity was not expressed with creatine kinase as the substrate. Cardiac tissue was shown to have a similar endogenous dethiolating activity. These results suggest that thioredoxin may play an important role in dethiolating specific proteins that might become S-thiolated during oxidative stress of cardiac tissue.

References

Sep 30, 1988·Biochemical and Biophysical Research Communications·R B Silverman, D L Nandi
Apr 22, 1987·Biochimica Et Biophysica Acta·M W Collison, J A Thomas
Aug 15, 1986·Analytical Biochemistry·J A Thomas, D Beidler
Jan 1, 1985·Annual Review of Biochemistry·A Holmgren
Jul 15, 1980·The Biochemical Journal·B Mannervik, K Axelsson

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Citations

Aug 12, 2000·Free Radical Biology & Medicine·E A KonorevN Hogg
Apr 19, 2000·The Biochemical Journal·S ReddyA Van Der Vliet
Jul 26, 2003·Antioxidants & Redox Signaling·Nikola J CostaMichael P Murphy
Jul 7, 2005·Antioxidants & Redox Signaling·Thomas R HurdMichael P Murphy
Sep 10, 1991·Biochemistry·J J MieyalB A Hocevar
Jun 25, 1991·Biochemistry·J J MieyalJ S Chung
Jun 1, 2006·Current Protocols in Toxicology·Aleksandra Filipovska, Michael P Murphy
Sep 24, 2016·The Journal of Biological Chemistry·Cécile MathieuFernando Rodrigues-Lima

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