Reduction of SOST gene promotes bone formation through the Wnt/β-catenin signalling pathway and compensates particle-induced osteolysis.

Journal of Cellular and Molecular Medicine
Zai Hang ZhangWei Xu

Abstract

The increase in bone resorption and/or the inhibition of bone regeneration caused by wear particles are the main causes of periprosthetic osteolysis. The SOST gene and Sclerostin, a protein synthesized by the SOST gene, are the characteristic marker of osteocytes and regulate bone formation and resorption. We aimed to verify whether the SOST gene was involved in osteolysis induced by titanium (Ti) particles and to investigate the effects of SOST reduction on osteolysis. The results showed osteolysis on the skull surface with an increase of sclerostin levels after treated with Ti particles. Similarly, sclerostin expression in MLO-Y4 osteocytes increased when treated with Ti particles in vitro. After reduction of SOST, local bone mineral density and bone volume increased, while number of lytic pores on the skull surface decreased and the erodibility of the skull surface was compensated. Histological analyses revealed that SOST reduction increased significantly alkaline phosphatase- (ALP) and osterix-positive expression on the skull surface which promoted bone formation. ALP activity and mineralization of MC3T3-E1 cells also increased in vitro when SOST was silenced, even if treated with Ti particles. In addition, Ti particles dec...Continue Reading

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Citations

Jan 2, 2021·Frontiers in Cell and Developmental Biology·Qian LiuGuangwei Wang
Nov 24, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jiaqi BaoLili Chen
Dec 18, 2020·Frontiers in Cell and Developmental Biology·Hidefumi Maeda
Oct 29, 2020·Archives of Oral Biology·Céline StutzOlivier Huck
Jul 3, 2021·International Journal of Molecular Sciences·Xia XuLifang Hu

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