Reduction of the monounsaturated fatty acid content of Escherichia coli results in increased resistance to oxidative damage

Microbiology
Gonzalo A PradenasClaudio C Vásquez

Abstract

Reactive oxygen species (ROSs) affect several macromolecules and cellular components in eukaryotic and prokaryotic cells. In this work, the effect of various ROS-generating compounds on the Escherichia coli membrane was studied. Membrane fatty acid profiles, oxidative damage levels and bacterial resistance to these toxicants were determined. Studies included wild-type cells as well as a strain exhibiting a modified monounsaturated fatty acid (MUFA) profile (accomplished by overexpressing the β-hydroxyacyl acyl carrier protein dehydratase-encoding gene, fabA). Levels of membrane MUFAs and oxidative damage markers decreased slightly upon toxicant exposure with a concomitant increase in cell resistance to these ROS-generating compounds. A direct relationship between MUFAs and lipid peroxidation was observed. The lower the MUFA the lower the peroxide levels, suggesting that MUFAs are targets for membrane lipid oxidation.

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Citations

Feb 1, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Waldo A Díaz-VásquezClaudio C Vásquez
Aug 31, 2013·BioMed Research International·Gonzalo A PradenasClaudio C Vásquez
Aug 26, 2015·MicrobiologyOpen·Robert A KanalyNatsuko Hamamura
May 1, 2012·Biochemical and Biophysical Research Communications·Claudia A ReinosoClaudio C Vásquez
Jul 23, 2014·Research in Microbiology·Roberto C Molina-QuirozClaudio C Vásquez
Dec 3, 2014·Archives of Biochemistry and Biophysics·Waldo A Díaz-VásquezClaudio C Vásquez
Feb 17, 2018·Applied Microbiology and Biotechnology·Tsz Wai NgKa Man Lai
Aug 6, 2019·Oxidative Medicine and Cellular Longevity·Claudia RodriguesAna Cipak Gasparovic
Mar 24, 2021·ACS Applied Materials & Interfaces·A Gala MorenaTzanko Tzanov

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