PMID: 9526046Apr 4, 1998Paper

Reduction of the plastoquinone pool by exogenous NADH and NADPH in higher plant chloroplasts. Characterization of a NAD(P)H-plastoquinone oxidoreductase activity

Biochimica Et Biophysica Acta
S CorneilleGilles Peltier

Abstract

Chlorophyll fluorescence measurements were performed on osmotically lysed potato chloroplasts in order to characterize the reactions involved in the dark reduction of photosynthetic inter-system chain electron carriers. Addition of NADH or NADPH to lysed chloroplasts increased the chlorophyll fluorescence level measured in the presence of a non-actinic light until reaching Fmax, thus indicating an increase in the redox state of the plastoquinone (PQ) pool. The fluorescence increase was more pronounced when the experiment was carried out under anaerobic conditions and was about 50% higher when NADH rather than NADPH was used as an electron donor. The NAD(P)H-PQ oxidoreductase reaction was inhibited by diphenylene iodonium, N-ethylmaleimide and dicoumarol, but insensitive to rotenone, antimycin A and piericidin A. By comparing the substrate specificity and the inhibitor sensitivity of this reaction to the properties of spinach ferredoxin-NADP+-reductase (FNR), we infer that FNR is not involved in the NAD(P)H-PQ oxidoreductase activity and conclude to the participation of rotenone-insensitive NAD(P)H-PQ oxidoreductase. By measuring light-dependent oxygen uptake in the presence of DCMU, methyl viologen and NADH or NADPH as an elect...Continue Reading

References

May 1, 1992·Plant Molecular Biology·M Sugiura
Jan 1, 1988·Trends in Biochemical Sciences·K OhyamaY Yamada
Nov 1, 1995·American Journal of Respiratory Cell and Molecular Biology·H InoueI F Ueki
Aug 1, 1996·Biochemical Society Transactions·L A SazanovP J Nixon
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G Peltier, G W Schmidt
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P Bennoun
Jun 15, 2013·Journal of Virological Methods·Hannah C JefferyLynn M Butler
Jun 1, 1993·Photosynthesis Research·Q J GroomD R Ort

❮ Previous
Next ❯

Citations

Aug 19, 2007·Photosynthesis Research·Taras K AntalAndrew B Rubin
Nov 16, 2004·Journal of Photochemistry and Photobiology. B, Biology·Maciej GarstkaMałgorzata Rosiak
Apr 19, 2002·Journal of Photochemistry and Photobiology. B, Biology·Nicole A Dijkman, Bernd M A Kroon
Sep 14, 2002·Annual Review of Plant Biology·Gilles Peltier, Laurent Cournac
Jun 12, 2013·Journal of Plant Physiology·Najoua MsiliniRobert Carpentier
Jan 25, 2011·Plant Physiology and Biochemistry : PPB·Jemâa EssemineRobert Carpentier
May 25, 2010·Plant Physiology and Biochemistry : PPB·Mercedes Martín, Bartolomé Sabater
Sep 13, 2008·The Plant Journal : for Cell and Molecular Biology·Atsushi TakabayashiFumihiko Sato
Jul 31, 2007·Plant, Cell & Environment·Dominique RumeauLaurent Cournac
May 20, 2015·Photosynthesis Research·Taras K AntalEsa Tyystjärvi
Jan 24, 2015·Biochimica Et Biophysica Acta·Alexander A Bulychev, Anna V Komarova
Nov 25, 2006·Biochimica Et Biophysica Acta·Fabrice RappaportPierre Joliot
Jan 7, 2016·Annual Review of Plant Biology·Gilles PeltierToshiharu Shikanai
Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·L CournacG Peltier
Jan 1, 2018·Functional Plant Biology : FPB·Anna V KomarovaAlexander A Bulychev
Sep 12, 2008·Plant Physiology·Marie GarmierA Harvey Millar

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.