PMID: 9173683Apr 1, 1996Paper

Reductive activation of 1,1-dichloro-1-fluoroethane (HCFC-141b) by phenobarbital- and pyridine-induced rat liver microsomal cytochrome P450

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
R TolandoM Manno

Abstract

1. During anaerobic reductive incubation of liver microsomes, from either the pyridine- or phenobarbital-treated rat, with 1,1-dichloro-1-fluoroethane (HCFC-141b) in the presence of a NADPH-regenerating system, a time- and dose-dependent formation of reactive metabolites was detected as indicated by a depletion of added exogenous glutathione. 2. A statistically significant, dose-dependent loss of both cytochrome P450 and microsomal haem was also observed under these experimental conditions. Furthermore, a statistically significant decrease of p-nitrophenol hydroxylase and pentoxyresorufin O-depentylase activity was measured in microsomes from the pyridine- and phenobarbital-induced rat, respectively indicating that both P4502E1 and P4502B undergo substrate-dependent inactivation. 3. Both reactive metabolite formation and P450 inactivation were almost completely inhibited by previous bubbling of the incubation mixture with carbon monoxide, indicating that interaction of the substrate with a free and reduced P450 haem iron is required for substrate bioactivation and enzyme loss. 4. The presence in the incubation mixture of the spin-trap N-t-butyl-alpha-phenylnitrone (PBN) and the carbene trap 2,3-dimethyl-2-butene (DMB) largely p...Continue Reading

References

Jan 6, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D R Koop
Mar 1, 1991·Chemical Research in Toxicology·J W Harris, M W Anders
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J W HarrisM W Anders
May 1, 1991·Biochemical Pharmacology·J W Harris, M W Anders
Jan 1, 1990·Pharmacology & Therapeutics·C S Lieber
Oct 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M TsutsumiC S Lieber
Jan 1, 1989·Toxicology·M J Garle, J R Fry
Dec 1, 1985·Environmental Health Perspectives·K H CheesemanT F Slater
Dec 1, 1988·Cancer Metastasis Reviews·J Longstreth
Dec 1, 1988·The New England Journal of Medicine·H R TaylorE A Emmett
Feb 1, 1980·Toxicology and Applied Pharmacology·R C JeeB R Brown
Jun 1, 1994·Risk Analysis : an Official Publication of the Society for Risk Analysis·A M JarabekJ N McDougal

❮ Previous
Next ❯

Citations

Nov 24, 1999·Reproductive Toxicology·N R PuigG A Elena
Feb 24, 2015·Journal of Pharmaceutical and Biomedical Analysis·Xiwei ZhengDavid S Hage
Jul 16, 2019·Chemistry : a European Journal·Yong Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.