Redundant and Diversified Roles Among Selected Arabidopsis thaliana EXO70 Paralogs During Biotic Stress Responses

Frontiers in Plant Science
Tamara PečenkováFatima Cvrčková

Abstract

The heterooctameric vesicle-tethering complex exocyst is important for plant development, growth, and immunity. Multiple paralogs exist for most subunits of this complex; especially the membrane-interacting subunit EXO70 underwent extensive amplification in land plants, suggesting functional specialization. Despite this specialization, most Arabidopsis exo70 mutants are viable and free of developmental defects, probably as a consequence of redundancy among isoforms. Our in silico data-mining and modeling analysis, corroborated by transcriptomic experiments, pinpointed several EXO70 paralogs to be involved in plant biotic interactions. We therefore tested corresponding single and selected double mutant combinations (for paralogs EXO70A1, B1, B2, H1, E1, and F1) in their two biologically distinct responses to Pseudomonas syringae, root hair growth stimulation and general plant susceptibility. A shift in defense responses toward either increased or decreased sensitivity was found in several double mutants compared to wild type plants or corresponding single mutants, strongly indicating both additive and compensatory effects of exo70 mutations. In addition, our experiments confirm the lipid-binding capacity of selected EXO70s, howe...Continue Reading

References

Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·N A BakerJ A McCammon
Dec 12, 2001·Nature Cell Biology·Serge MoskalenkoMichael A White
Aug 16, 2002·Current Opinion in Plant Biology·Ana M Laxalt, Teun Munnik
Apr 12, 2003·Cell Biology International·M EliasV Zarsky
Mar 24, 2004·International Review of Cytology·Shu-Chan HsuWei Guo
May 12, 2004·Current Opinion in Plant Biology·Xuemin Wang
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Jan 24, 2007·The Plant Journal : for Cell and Molecular Biology·Ziguo ZhangHans Thordal-Christensen
May 23, 2007·Nucleic Acids Research·Markus Wiederstein, Manfred J Sippl
May 22, 2008·The Plant Cell·Michal HálaViktor Zárský
Jun 6, 2009·The New Phytologist·Viktor ŽárskýMichal Hála
Jan 1, 2008·Advances in Bioinformatics·Tomas HruzPhilip Zimmermann
May 27, 2010·Nucleic Acids Research·M L HekkelmanG Vriend
Sep 28, 2010·The Plant Cell·Matyás FendrychViktor Zársky
Jan 5, 2011·Journal of Experimental Botany·Tamara PecenkováViktor Zársky
Jul 25, 2012·Frontiers in Plant Science·Fatima CvrčkováViktor Zárský
Nov 21, 2012·The Plant Journal : for Cell and Molecular Biology·Edita Janková DrdováViktor Zárský
Jan 4, 2013·Molecular Biology of the Cell·Matyás FendrychViktor Zársky
Aug 1, 2013·Physiological Reviews·Tamas Balla
Aug 21, 2013·Developmental Cell·Yuting ZhaoWei Guo
Nov 20, 2013·Current Opinion in Plant Biology·Viktor ZárskýTamara Pečenková
Apr 4, 2015·Biochimica Et Biophysica Acta·Roman PleskotMartin Potocký
Jul 15, 2015·Molecular Reproduction and Development·Johannes SchindelinKevin W Eliceiri
Oct 9, 2015·The Journal of Biological Chemistry·Michelle L DubukeMary Munson
May 6, 2016·Frontiers in Cell and Developmental Biology·Magdalena Martin-UrdirozIsabelle Jourdain
Jun 21, 2016·Current Protocols in Bioinformatics·Benjamin Webb, Andrej Sali
Nov 2, 2016·The New Phytologist·Nemanja VukašinovićViktor Žárský
Dec 3, 2016·Frontiers in Plant Science·Rowan van WerschYuelin Zhang
Dec 8, 2016·Structure·Shuguang YuanHorst Vogel

❮ Previous
Next ❯

Citations

Jul 25, 2021·International Journal of Molecular Sciences·Vedrana MarkovićViktor Žárský

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE18986

Methods Mentioned

BETA
GTPases
gene knock-out
genotyping
PCR
chip
two hybrid

Software Mentioned

ImageJ
Genevestigator
HR
Excel
ggplot2
Genevestigator gene expression
Pymol
DOPE
BioEdit
Inkscape

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Alex Chun KoonHo Yin Edwin Chan
Cellular & Molecular Biology Letters
Wenqing MaMiao Yin
© 2021 Meta ULC. All rights reserved