Redundant control of rereplication in fission yeast

Proceedings of the National Academy of Sciences of the United States of America
V GopalakrishnanT J Kelly

Abstract

The initiation of DNA replication at replication origins in eukaryotic cells is tightly controlled to ensure that the genome is duplicated only once each cell cycle. We present evidence that in fission yeast, independent regulation of two essential components of the initiation complex, Cdc18 and Cdt1, contributes to the prevention of reinitiation of DNA replication. Cdc18 is negatively controlled by cyclin-dependent kinase (CDK) phosphorylation, but low level expression of a mutant form of Cdc18 lacking CDK phosphorylation sites (Cdc18(CDK)) is not sufficient to induce rereplication. Similar to Cdc18, Cdt1 is expressed periodically in the cell cycle, accumulating in the nucleus in G(1) and declining in G(2). When Cdt1 is expressed constitutively from an ectopic promoter, it accumulates in the nucleus throughout the cell cycle but does not promote reinitiation. However, constitutive expression of Cdt1, together with Cdc18(CDK), is sufficient to induce extra rounds of DNA replication in the absence of mitosis. Significantly greater levels of rereplication can be induced by coexpression of Cdc18(CDK) and a Cdt1 mutant lacking a conserved C-terminal motif. In contrast, uncontrolled DNA replication does not occur when either mutant ...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·S R WenteG Blobel
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J DemeterS Sazer
Jun 25, 1993·Nucleic Acids Research·S L Forsburg
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·M Muzi FalconiT J Kelly
Jun 1, 1997·Current Opinion in Cell Biology·P V Jallepalli, T J Kelly
Jun 3, 1997·Gene·S L Forsburg, D A Sherman
Sep 26, 1997·The Journal of Biological Chemistry·Y Ishimi
Nov 14, 1997·Genes & Development·P V JallepalliT J Kelly
Mar 14, 1998·Molecular Biology of the Cell·A Lopez-GironaP Russell
Jul 11, 1998·Cell·T J McGarry, M W Kirschner
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·P V JallepalliT J Kelly
Aug 5, 1998·Biochimica Et Biophysica Acta·S E Kearsey, K Labib
Aug 15, 1998·The Journal of Biological Chemistry·G W Brown, T J Kelly
Sep 22, 1999·Molecular and Cellular Biology·Y OgawaH Masukata
Aug 31, 2000·Annual Review of Biochemistry·T J Kelly, G W Brown
Dec 23, 2000·Science·J A WohlschlegelA Dutta
Aug 4, 2001·Molecular and Cellular Biology·A VasJ Leatherwood

❮ Previous
Next ❯

Citations

Dec 20, 2002·Journal of Structural Biology·Susan A GerbiJohn M Waggener
Aug 4, 2012·ACS Chemical Neuroscience·Zhen HeMerle G Paule
Jun 13, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hideo Nishitani, Zoi Lygerou
Aug 7, 2009·The Journal of Biological Chemistry·Yadushyla Narasimhachar, Martine Coué
Nov 13, 2004·Molecular Biology of the Cell·Brian M Green, Joachim J Li
Jan 11, 2013·Molecular Biology of the Cell·Hui HuaStephen E Kearsey
Feb 1, 2011·Journal of Molecular Cell Biology·Lan N Truong, Xiaohua Wu
Apr 16, 2011·Nucleic Acids Research·Estrella GuarinoStephen E Kearsey
May 10, 2012·Nucleic Acids Research·Manpreet KaurSandeep Saxena
Jan 22, 2013·Cold Spring Harbor Perspectives in Biology·Tarek AbbasAnindya Dutta
Aug 19, 2004·The Plant Cell·María del Mar CastellanoCrisanto Gutierrez
Jan 27, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazumasa YoshidaYumiko Kubota
May 31, 2012·Molecular and Cellular Biology·Zhen ShenSupriya G Prasanth
Jun 5, 2002·Annual Review of Biochemistry·Stephen P Bell, Anindya Dutta
Dec 25, 2007·BMC Molecular Biology·Katie L MickleJanet Leatherwood
Aug 26, 2010·Journal of Cell Science·Lee KiangPaul Nurse
Sep 15, 2010·PLoS Genetics·Queying Ding, David M MacAlpine
Feb 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nilangshu DasDhruba K Chattoraj
Dec 18, 2004·Mutation Research·Sandeep Saxena, Anindya Dutta
Dec 6, 2005·European Journal of Cell Biology·Baoqing GuoHoyun Lee
Sep 24, 2004·Current Biology : CB·John F X Diffley
Apr 30, 2003·Molecular Cell·Cyrus VaziriAnindya Dutta
Jan 18, 2017·Critical Reviews in Biochemistry and Molecular Biology·Matthew W ParkerJames M Berger
Dec 16, 2003·The Journal of Biological Chemistry·Jeanette Gowen CookJoseph R Nevins
May 10, 2017·Molecular Cancer Research : MCR·Tara H W DobsonVidya Gopalakrishnan
Nov 4, 2004·Journal of Cell Science·Kathryn Leigh EwardGareth H Williams
Jul 14, 2021·Chromosoma·Yutaka YamamotoSusan A Gerbi
Mar 9, 2007·Genes & Development·Emily E Arias, Johannes C Walter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.