Reelin and cofilin cooperate during the migration of cortical neurons: a quantitative morphological analysis

Development
Xuejun ChaiMichael Frotscher

Abstract

In reeler mutant mice, which are deficient in reelin (Reln), the lamination of the cerebral cortex is disrupted. Reelin signaling induces phosphorylation of LIM kinase 1, which phosphorylates the actin-depolymerizing protein cofilin in migrating neurons. Conditional cofilin mutants show neuronal migration defects. Thus, both reelin and cofilin are indispensable during cortical development. To analyze the effects of cofilin phosphorylation on neuronal migration we used in utero electroporation to transfect E14.5 wild-type cortical neurons with pCAG-EGFP plasmids encoding either a nonphosphorylatable form of cofilin 1 (cofilin(S3A)), a pseudophosphorylated form (cofilin(S3E)) or wild-type cofilin 1 (cofilin(WT)). Wild-type controls and reeler neurons were transfected with pCAG-EGFP. Real-time microscopy and histological analyses revealed that overexpression of cofilin(WT) and both phosphomutants induced migration defects and morphological abnormalities of cortical neurons. Of note, reeler neurons and cofilin(S3A)- and cofilin(S3E)-transfected neurons showed aberrant backward migration towards the ventricular zone. Overexpression of cofilin(S3E), the pseudophosphorylated form, partially rescued the migration defect of reeler neuro...Continue Reading

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Citations

Feb 1, 2018·International Journal of Molecular Sciences·Matthias SchlauCarsten Theiss
Oct 28, 2019·EMBO Reports·Durga Praveen MekaFroylan Calderon de Anda
Jul 26, 2016·Frontiers in Cellular Neuroscience·Hans H Bock, Petra May
Apr 8, 2017·Journal of Veterinary Science·Guohong LiShanting Zhao
Jun 8, 2017·Frontiers in Cellular Neuroscience·Michael FrotscherXuejun Chai
Jun 3, 2017·Frontiers in Cellular Neuroscience·Anja KonietznyMarina Mikhaylova
May 17, 2017·Frontiers in Cell and Developmental Biology·Yuki Hirota, Kazunori Nakajima
Aug 22, 2018·Journal of Molecular Histology·Mengmeng LiuShanting Zhao
Apr 23, 2020·Frontiers in Neuroscience·Xiaoyan ZhuShanting Zhao
Sep 10, 2020·Frontiers in Molecular Neuroscience·Xuejun ChaiShanting Zhao
Mar 8, 2017·Neurogenesis·Xuejun Chai, Michael Frotscher
Jan 2, 2019·Brain Plasticity·Youngshik Choe, Samuel J Pleasure
Nov 16, 2016·Histochemistry and Cell Biology·Jiongfang XieShanting Zhao
Dec 9, 2020·Computer Methods in Biomechanics and Biomedical Engineering·Hironori TakedaTaiji Adachi
Dec 8, 2020·The International Journal of Neuropsychopharmacology·Xiaoyan ZhuShanting Zhao
Jun 3, 2020·Molecular and Cellular Neurosciences·Yves Jossin
Sep 7, 2021·Frontiers in Molecular Neuroscience·Catarina OrcinhaCarola A Haas

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