Reexamination of the role of the amino terminus of SecA in promoting its dimerization and functional state.

Journal of Bacteriology
Sanchaita DasDonald B Oliver

Abstract

The SecA nanomotor promotes protein translocation in eubacteria by binding both protein cargo and the protein-conducting channel and by undergoing ATP-driven conformation cycles that drive this process. There are conflicting reports about whether SecA functions as a monomer or dimer during this dynamic process. Here we reexamined the roles of the amino and carboxyl termini of SecA in promoting its dimerization and functional state by examining three secA mutants and the corresponding proteins: SecADelta8 lacking residues 2 to 8, SecADelta11 lacking residues 2 to 11, and SecADelta11/N95 lacking both residues 2 to 11 and the carboxyl-terminal 70 residues. We demonstrated that whether SecADelta11 or SecADelta11/N95 was functional for promoting cell growth depended solely on the vivo level of the protein, which appeared to govern residual dimerization. All three SecA mutant proteins were defective for promoting cell growth unless they were highly overproduced. Cell fractionation revealed that SecADelta11 and SecADelta11/N95 were proficient in membrane association, although the formation of integral membrane SecA was reduced. The presence of a modestly higher level of SecADelta11/N95 in the membrane and the ability of this protein t...Continue Reading

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Citations

Feb 26, 2009·The Journal of Biological Chemistry·Nathan W RigelMiriam Braunstein
Nov 8, 2011·Journal of Bacteriology·Lorry M GradyDonald B Oliver
May 7, 2013·Biophysics Reviews·Daniel Some
May 7, 2010·Molecular Microbiology·Marios Frantzeskos Sardis, Anastassios Economou
Dec 18, 2013·Molecular Cell·Giorgos GouridisAnastassios Economou
Feb 15, 2011·Journal of Molecular Biology·Andy J WoworJames L Cole
Apr 8, 2014·Biochemical and Biophysical Research Communications·Hongyun WangPhang C Tai
Dec 3, 2014·Biochemical and Biophysical Research Communications·Bing NaPhang C Tai
Nov 2, 2016·Molecular Microbiology·Tithi BanerjeeDonald Oliver
Aug 12, 2017·Proceedings of the National Academy of Sciences of the United States of America·Qi ZhangIshita Mukerji
Sep 20, 2017·Scientific Reports·Aliakbar Khalili YazdiBrian H Shilton
Nov 5, 2019·Biochimica Et Biophysica Acta. Biomembranes·Guillaume Roussel, Stephen H White
Aug 4, 2021·Physical Chemistry Chemical Physics : PCCP·Fabian SoltermannPhilipp Kukura
Jul 28, 2021·The FEBS Journal·Michael KamelAlexej Kedrov
May 3, 2014·Biochemistry·Andy J WoworJames L Cole

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