Reexpression of vimentin in differentiated neuroblastoma cells enhances elongation of axonal neurites

Journal of Neuroscience Research
Maya DubeyT B Shea

Abstract

Vimentin (Vm) is initially expressed by early neuronal precursors in situ and in culture. Vm is essential for neuritogenesis at least in culture and is gradually replaced by neurofilaments (NFs) because of down-regulation of Vm expression. This period is accompanied by a slowing of axonal elongation. We examined whether continued expression of Vm could foster continued axonal elongation. NB2a/d1 cells differentiated with dibutyryl cAMP were transfected with constructs expressing Vm or the middle-molecular-weight NF subunit (NF-M) each conjugated to green fluorescent protein (GFP). Axonal neurites of cells expressing GFP-Vm were 30% longer than those of nonexpressing cells, or cells expressing GFP-M, and exhibited a decrease in neurite caliber. Expression of GFP-M did not enhance axonal neurite length but significantly increased caliber. These findings provide further evidence of a role for Vm in axonal outgrowth. Culturing of nontransfected cells on laminin increased neurite length, but cells expressing GFP-Vm demonstrated an equivalent increase whether cultured on laminin or culture plastic. Axonal neurites of cells expressing GFP-Vm turned to avoid a nonfavorable substrate (nitrocellulose), but culturing of these cells on nit...Continue Reading

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Citations

Jul 16, 2010·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·A Granata, T T Warner
Apr 24, 2008·PloS One·Cory TothMinh Dang Nguyen
Jan 20, 2006·The European Journal of Neuroscience·Arianna Del SignorePaola Paggi
Oct 11, 2005·Trends in Cell Biology·Brian T HelfandRobert D Goldman
Apr 14, 2016·Scientific Reports·Cristina Velez-delValleWalid Kuri-Harcuch
Mar 16, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Thanh D DoJonathan V Sweedler
Feb 28, 2008·The Journal of Biological Chemistry·Su Yeon ShimMinh Dang Nguyen
Apr 14, 2020·Cellular and Molecular Life Sciences : CMLS·Roberta RomanoCecilia Bucci
Mar 27, 2020·Frontiers in Cell and Developmental Biology·Samuele LaudaniFulvia Sinatra
Jul 29, 2020·Frontiers in Cellular Neuroscience·William RodemerMichael E Selzer
Aug 14, 2020·Experimental Biology and Medicine·Sheron Campos CogoSelene Elifio-Esposito

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