Reference genes for accessing differential expression among developmental stages and analysis of differential expression of OBP genes in Anastrepha obliqua

Scientific Reports
Aline Minali NakamuraReinaldo Alves de Brito

Abstract

The West Indian fruit fly, Anastrepha obliqua, is an important agricultural pest in the New World. The use of pesticide-free methods to control invasive species such as this reinforces the search for genes potentially useful in their genetic control. Therefore, the study of chemosensory proteins involved with a range of responses to the chemical environment will help not only on the understanding of the species biology but may also help the development of environmentally friendly pest control strategies. Here we analyzed the expression patterns of three OBP genes, Obp19d_2, Obp56a and Obp99c, across different phases of A. obliqua development by qPCR. In order to do so, we tested eight and identified three reference genes for data normalization, rpl17, rpl18 and ef1a, which displayed stability for the conditions here tested. All OBPs showed differential expression on adults and some differential expression among adult stages. Obp99c had an almost exclusive expression in males and Obp56a showed high expression in virgin females. Thereby, our results provide relevant data not only for other gene expression studies in this species, as well as for the search of candidate genes that may help in the development of new pest control str...Continue Reading

References

Mar 1, 1997·Insect Biochemistry and Molecular Biology·M F Wolfner
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·U Tram, M F Wolfner
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 7, 2004·Biochemical and Biophysical Research Communications·Aleksandar RadonićAndreas Nitsche
Aug 25, 2004·Current Biology : CB·Lisa A McGrawMariana F Wolfner
Jun 28, 2005·Insect Biochemistry and Molecular Biology·Jonathan Bohbot, Richard G Vogt
Jun 21, 2006·Cellular and Molecular Life Sciences : CMLS·R Benton
Nov 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Peter E CookScott L O'Neill
Dec 12, 2007·Evolution; International Journal of Organic Evolution·Kurt A McKean, Leonard Nunney
Sep 10, 1981·Nature·R G Vogt, L M Riddiford
May 27, 2008·Biochemical and Biophysical Research Communications·Sha LiPaolo Pelosi
Oct 10, 2009·PLoS Genetics·Shanshan ZhouRobert R H Anholt
May 25, 2011·Genes, Brain, and Behavior·S SwarupR R H Anholt
Nov 15, 2011·Insect Biochemistry and Molecular Biology·Marc F ScheteligAlfred M Handler
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley
Jul 23, 2013·International Journal of Molecular Sciences·Weiwei ZhengHongyu Zhang

❮ Previous
Next ❯

Citations

Oct 19, 2016·Scientific Reports·Anoeska Agatha Alida van de Moosdijk, Renée van Amerongen
Feb 25, 2017·Insect Molecular Biology·S Chahad-EhlersR A de Brito
Oct 14, 2017·Journal of Economic Entomology·Meire Menezes BassanFrancisco de Assis Alves Moura O Filho
Aug 4, 2018·International Journal of Molecular Sciences·Yuning HuYan Wu
Aug 28, 2020·Frontiers in Physiology·Clancy A ShortDaniel A Hahn
May 21, 2017·Scientific Reports·Emeline Boni CampaniniReinaldo Alves de Brito
May 6, 2021·Insects·Francesca ScolariLucie Vaníčková
Jul 14, 2021·Journal of Insect Physiology·Nancy de Fátima Chaves RegoReinaldo Alves de Brito
Mar 15, 2019·Journal of Agricultural and Food Chemistry·Bowen TangChunni Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
electrophoresis

Software Mentioned

edgeR
CFX Manager
BLAST
GraphPad
NormFinder
OligoAnalyzer
BestKeeper
Prism
MAFFT
RefFinder

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.