Reference genes for real-time PCR quantification of microRNAs and messenger RNAs in rat models of hepatotoxicity.

PloS One
María Noelia LardizábalLuis María Veggi

Abstract

Hepatotoxicity is associated with major changes in liver gene expression induced by xenobiotic exposure. Understanding the underlying mechanisms is critical for its clinical diagnosis and treatment. MicroRNAs are key regulators of gene expression that control mRNA stability and translation, during normal development and pathology. The canonical technique to measure gene transcript levels is Real-Time qPCR, which has been successfully modified to determine the levels of microRNAs as well. However, in order to obtain accurate data in a multi-step method like RT-qPCR, the normalization with endogenous, stably expressed reference genes is mandatory. Since the expression stability of candidate reference genes varies greatly depending on experimental factors, the aim of our study was to identify a combination of genes for optimal normalization of microRNA and mRNA qPCR expression data in experimental models of acute hepatotoxicity. Rats were treated with four traditional hepatotoxins: acetaminophen, carbon tetrachloride, D-galactosamine and thioacetamide, and the liver expression levels of two groups of candidate reference genes, one for microRNA and the other for mRNA normalization, were determined by RT-qPCR in compliance with the ...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J Armendariz-BorundaR Raghow
Feb 11, 1999·The Annals of Pharmacotherapy·D K Farver, M N Lavin
Apr 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K Selim, N Kaplowitz
Jan 5, 2000·Journal of Biotechnology·O ThellinE Heinen
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Apr 16, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Alexandra N HeinlothRichard S Paules
Apr 5, 2005·Toxicologic Pathology·Harihara M Mehendale
May 12, 2005·Mutation Research·Timothy W Gant, Shu-Dong Zhang
Nov 30, 2005·Nucleic Acids Research·Caifu ChenKarl J Guegler
Dec 6, 2005·BMC Developmental Biology·Karen GoossensLuc J Peelman
Mar 3, 2006·Genes & Development·Marco Antonio Valencia-SanchezRoy Parker
May 27, 2006·Endocrine-related Cancer·P PallanteA Fusco
Jun 17, 2006·Biochemical and Biophysical Research Communications·Masuhiro NishimuraShinsaku Naito
Apr 5, 2007·Nature Protocols·Tania NolanStephen A Bustin
Feb 5, 2008·Synapse·Birgit Egeskov BonefeldGregers Wegener
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Aug 20, 2008·Journal of Neuroscience Research·Naomi L CookCorinna van den Heuvel
Sep 10, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Makoto MiyamotoRyoetsu Imai
Jan 24, 2009·Nature Reviews. Molecular Cell Biology·Vamsi K Gangaraju, Haifan Lin
Feb 19, 2009·Nucleic Acids Research·Steve LefeverUNKNOWN RDML consortium
Feb 21, 2009·BMC Bioinformatics·Vasyl PihurSomnath Datta
Feb 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kai WangDavid J Galas
Apr 11, 2009·World Journal of Gastroenterology : WJG·Xian-Ming Chen
Apr 28, 2009·The Plant Cell·Ivo Rieu, Stephen J Powers
Jun 18, 2009·Genome Biology·Pieter MestdaghJo Vandesompele
Dec 5, 2009·World Journal of Gastroenterology : WJG·Shashi BalaGyongyi Szabo
Dec 9, 2009·Methods : a Companion to Methods in Enzymology·Stefaan DerveauxJan Hellemans
Mar 27, 2010·Molecular Biotechnology·Gai-Ping Wang, Cun-Shuan Xu

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Citations

Jan 7, 2014·Environmental Toxicology and Pharmacology·María Noelia LardizábalLuis María Veggi
Feb 13, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jin WangSubrata Sen
Apr 2, 2014·Thyroid : Official Journal of the American Thyroid Association·Paola B NavasMaría B Hapon
Mar 19, 2013·Immunopharmacology and Immunotoxicology·Irmak FerahElif Cadirci
Apr 16, 2015·International Journal of Molecular Sciences·Xin ZhuWu-Ying Chu
Oct 31, 2014·Expert Review of Molecular Diagnostics·Kira S Sheinerman, Samuil Umansky
Oct 3, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mohammad Hossein AnsariAmaneh Mohammadi Roushandeh
Jul 23, 2014·Asian-Australasian Journal of Animal Sciences·Yu ZhangYun-Hai Zhang
Jun 13, 2015·BMC Developmental Biology·Mahdi MahdipourBernard A J Roelen
Dec 30, 2016·Forensic Science, Medicine, and Pathology·Ye-Hui LvLong Chen
Mar 27, 2019·Development·Estefanía Sánchez-VásquezPablo H Strobl-Mazzulla
Mar 8, 2018·International Journal of Molecular Medicine·Sheng WangXiongwen Lv
Sep 4, 2020·Viruses·Beata Hukowska-SzematowiczBeata Burzyńska
Jan 13, 2019·Cancers·Carla SoléCharles H Lawrie

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Methods Mentioned

BETA
PCR
Assay
light microscopy
electrophoresis

Software Mentioned

R
NormFinder
MxPro
BestKeeper
geNorm
Excel
Keeper
Best
RG
RankAggreg

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