Refined crystal structure of porcine class Pi glutathione S-transferase (pGST P1-1) at 2.1 A resolution

Journal of Molecular Biology
H W DirrR Huber


The crystal structure of class Pi glutathione S-transferase from porcine lung (pGST P1-1) in complex with glutathione sulphonate has been refined at 2.11 A resolution, to a crystallographic R-factor of 16.5% for 21, 165 unique reflections. The refined structure includes 3314 protein atoms, 46 inhibitor (glutathione sulphonate) atoms and 254 water molecules. The model shows good stereochemistry, with root-mean-square deviations from ideal bond lengths and bond angles of 0.011 A and 2.8 degrees, respectively. The estimated root-mean-square co-ordinate error is 0.2 A. The protein is a dimer assembled from identical subunits of 207 amino acid residues. The tertiary structure of the pGST P1 subunit is organized as two domains, the N-terminal domain (domain I, residues 1 to 74) and the larger C-terminal domain (domain II, residues 81 to 207). Glutathione sulphonate, a competitive inhibitor, binds to the G-site region (i.e. the glutathione-binding region) of the active site located on each subunit. Each G-site is, however, structurally dependent of the neighbouring subunit as structural elements forming a fully functional G-site are provided by both subunits, with domain I as the major supporting framework. A number of direct and wate...Continue Reading


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May 3, 2005·Journal of Molecular Modeling·Sivaramakrishnan Thirumalai NathanKothandapani Balaraman
Feb 16, 2012·Bioorganicheskaia khimiia·P N BoltovetsB A Snopok
Oct 1, 1996·Journal of Biomolecular Structure & Dynamics·Y J Zheng, R L Ornstein
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Mar 24, 1999·Archives of Biochemistry and Biophysics·N E PettigrewR F Colman
Jun 5, 2002·Insect Biochemistry and Molecular Biology·M F Rosa de LimaA Masuda
Apr 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T Kunze
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