Refined Crystal Structure of Samia cynthia ricini Silk Fibroin Revealed by Solid-State NMR Investigations

Biomacromolecules
T AsakuraAkira Naito

Abstract

Samia cynthia ricini is one of the wild silkworms and its silk fibroin (SF) consists of alternatively repeating poly-l-alanine (PLA) sequences as crystalline domain and glycine-rich sequences as noncrystalline domain; the structure is similar to those of spider silk and other wild silkworm silks. In this paper, we proposed a new staggered model for the packing arrangement of the PLA sequence through the use of the Cambridge Serial Total Energy Package program and a comparison of the observed and calculated chemical shifts of the PLA sequence with the Gauge Including Projector Augmented Wave method. The new model was supported by the interatomic distance information from the cross peaks of Ala Cβ dipolar-assisted rotational resonance (DARR) spectrum of the PLA sequences in S. c. ricini SF fiber. In addition, three13C NMR peaks observed in the β-sheet region were assigned to the carbons with different environments in the same model, but not assigned to different β-sheet structures.

References

Nov 14, 1966·Journal of Molecular Biology·S Arnott, A J Wonacott
May 26, 1999·International Journal of Biological Macromolecules·Y TakahashiK Yuzuriha
May 26, 1999·International Journal of Biological Macromolecules·C Y HayashiR V Lewis
Jun 28, 2000·Nucleic Acids Research·C Z ZhouZ G Li
Aug 3, 2001·Insect Biochemistry and Molecular Biology·A DattaS C Kundu
Jul 26, 2002·Journal of the American Chemical Society·Tetsuo AsakuraAnne S Ulrich
Jan 1, 1955·Biochimica Et Biophysica Acta·R E MARSHL PAULING
Jul 21, 2006·The Journal of Physical Chemistry. B·K YamauchiT Asakura
Jul 3, 2008·Journal of the American Chemical Society·Gregory P HollandJeffery L Yarger
Dec 17, 2008·Journal of the American Chemical Society·Vipin AgarwalBernd Reif
Mar 26, 2009·The Journal of Chemical Physics·Marvin J BayroRobert G Griffin
Jan 1, 2007·Progress in Polymer Science·Charu Vepari, David L Kaplan
Oct 30, 2009·Chemical Communications : Chem Comm·Chengjie FuVollrath Fritz
Jan 10, 2012·Angewandte Chemie·Tetsuo AsakuraMike P Williamson
Mar 8, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Tetsuo AsakuraJeffery L Yarger
Aug 22, 2013·Acta Biomaterialia·Olena TokarevaDavid L Kaplan
Sep 9, 2015·The Journal of Experimental Biology·Maxime Boulet-AudetChris Holland
Jun 10, 2016·Scientific Reports·Ali D MalayKeiji Numata

❮ Previous
Next ❯

Citations

Aug 4, 2018·Macromolecular Rapid Communications·Meghan McGillDavid L Kaplan
Jun 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tetsuo Asakura
Oct 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kelvin O MosetiYasumoto Nakazawa
Feb 9, 2018·The Journal of Physical Chemistry. B·Akira NaitoTetsuo Asakura

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.