Refined structure of the pyruvoyl-dependent histidine decarboxylase from Lactobacillus 30a

Journal of Molecular Biology
T GallagherM L Hackert

Abstract

The crystal structure of the pyruvoyl-dependent histidine decarboxylase from Lactobacillus 30a has been refined to an R-value of 0.15 (for the 5.0 to 2.5 A resolution shell) and 0.17 (for the 10.0 to 2.5 A resolution shell). A description of the overall structure is presented, focusing on secondary structure and subunit association. The enzyme is a hexamer of alpha beta subunits. Separate alpha and beta-chains arise from an autocatalytic cleavage reaction between two serine residues, which results in the pyruvoyl cofactor. The central core of the alpha beta subunit is a beta-sandwich which consists of two face-to-face three-stranded antiparallel beta-sheets, flanked by alpha-helices on each side. The beta-sandwich creates a stable fold that allows conformational strain to be introduced across an internal cleavage region between the alpha and beta chains and places the pyruvoyl cofactor in a position for efficient electron withdrawal from the substrate. Three alpha beta subunits are related by a molecular three-fold symmetry axis to form a trimer whose interfaces have complementary surfaces and extensive molecular interactions. Each of the interfaces contains an active site and a solvent channel that leads from the active site t...Continue Reading

Citations

Dec 10, 2009·Amino Acids·Shridhar Bale, Steven E Ealick
Apr 18, 1998·Nature Structural Biology·F B Perler
Aug 30, 2008·Critical Reviews in Food Science and Nutrition·José María LandeteRosario Muñoz
Mar 5, 2005·Applied and Environmental Microbiology·Patrick M LucasAline Lonvaud-Funel
Apr 5, 2014·Acta Crystallographica. Section D, Biological Crystallography·Michael E WebbChris Abell
Dec 25, 2010·Biochimica Et Biophysica Acta·John P RichardAna Rios
Jul 29, 2004·Proteins·Adi BarzilaiRuth Nussinov
Jan 26, 2010·Essays in Biochemistry·Anthony E Pegg
Aug 31, 2000·Annual Review of Biochemistry·H Paulus
Mar 4, 2008·Journal of Molecular Biology·Denny G A JohanssonTorleif Härd
Jul 25, 2000·Chemistry & Biology·N M Okeley, W A van der Donk
Nov 14, 1998·Journal of Molecular Biology·S H DoneR E Hubbard
Jun 1, 1997·Proteins·A V Efimov
May 20, 2004·The Journal of Biological Chemistry·Angela V TomsSteven E Ealick
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Shridhar BaleSteven E Ealick
Jul 20, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Steven C DavisCarl A Gruetter
Aug 23, 1996·The Journal of Biological Chemistry·T W HamelryckR Loris
Nov 14, 1997·The Journal of Biological Chemistry·H XiongA E Pegg
Mar 13, 2001·Journal of Molecular Biology·E SchelpJ D Robertus
Oct 2, 2001·Current Opinion in Chemical Biology·L Poppe
Jul 25, 2000·European Journal of Medicinal Chemistry·F S TahanejadM Mahmoudian
Sep 3, 2003·Biochemical and Biophysical Research Communications·V Badireenath Konkimalla, Nagasuma Chandra

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