Refined structure of villin 14T and a detailed comparison with other actin-severing domains

Protein Science : a Publication of the Protein Society
M A MarkusG Wagner

Abstract

Villin 14T is the amino terminal actin monomer binding domain from the actin-severing and bundling protein villin. Its structure has been determined in solution using heteronuclear multidimensional nuclear magnetic resonance (NMR) spectroscopy (Markus MA, Nakayama T, Matsudaira P, Wagner G. 1994. Solution structure of villin 14T, a domain conserved among actin-severing proteins. Protein Science 3:70-81). An additional nuclear Overhauser effect (NOE) spectroscopy data set, acquired using improved gradient techniques, and further detailed analysis of existing data sets, produced an additional 601 NOE restraints for structure calculations. The overall fold does not change significantly with the additional NOE restraints but the definition of the structure is improved, as judged by smaller deviations among an ensemble of calculated structures that adequately satisfy the NMR restraints. Some of the side chains, especially those in the hydrophobic core of the domain, are much more defined. This improvement in the detail of the solution structure of villin 14T makes it interesting to compare the structure with the crystal structure of gelsolin segment 1, which shares 58% sequence identity with villin 14T, in an effort to gain insight ...Continue Reading

References

Jun 1, 1992·Protein Science : a Publication of the Protein Society·S G HybertsG Wagner
Mar 1, 1992·The Journal of Cell Biology·J FinidoriD Louvard
Jul 1, 1992·Journal of Biomolecular NMR·T SzyperskiK Wüthrich
Sep 6, 1990·Nature·K C HolmesW Kabsch
May 1, 1989·The Journal of Cell Biology·D J KwiatkowskiH L Yin
May 1, 1988·The Journal of Cell Biology·J Bryan
Jul 15, 1988·Cell·P Matsudaira, P Janmey
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W L BazariY Ahmed
Jun 17, 1988·Science·J S Richardson, D C Richardson
Sep 1, 1995·Journal of Structural Biology·A McGough, M Way
Mar 17, 1995·Journal of Molecular Biology·A SchnuchelT A Holak
Jan 1, 1994·Protein Science : a Publication of the Protein Society·M A MarkusG Wagner
Nov 1, 1993·Proteins·M W MacArthur, J M Thornton
Dec 1, 1993·Current Biology : CB·M Way, P Matsudaira

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Citations

Jan 9, 2003·Current Opinion in Cell Biology·Steven J Winder
Apr 1, 1997·Current Opinion in Cell Biology·A C Newton
Mar 4, 1998·Current Opinion in Cell Biology·Y A PuiusS C Almo
Jan 28, 2012·The Journal of Physical Chemistry. B·David ShirvanyantsNikolay V Dokholyan
Aug 3, 2005·Nature Reviews. Molecular Cell Biology·Phyllis I Hanson, Sidney W Whiteheart
Mar 20, 2004·Nature Reviews. Microbiology·Pongpan LaksanalamaiFrank T Robb
Jun 6, 2009·The Journal of Biological Chemistry·Hui WangRobert C Robinson
Jan 22, 2005·The Plant Cell·Shanjin HuangChristopher J Staiger
Sep 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·S L KazmirskiA R Fersht
Oct 25, 2013·Cytoskeleton·Umesh GhoshdastiderRobert C Robinson
Jun 12, 2013·Cytoskeleton·Shalini NagLeslie D Burtnick
Dec 17, 2008·Structure·Cheri M HamptonKenneth A Taylor
Sep 28, 2013·Biochemistry·Stanislav O FedechkinSerge L Smirnov
Feb 14, 2020·Journal of Cell Science·Wanying ZhaoShanjin Huang
Mar 25, 1999·Proteins·J BadgerS Szalma
Aug 15, 2001·American Journal of Physiology. Cell Physiology·A PanebraS Khurana
Feb 17, 1999·Biochimica Et Biophysica Acta·M Van TroysC Ampe

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