PMID: 9650072Jul 3, 1998Paper

Refinement of 3D structure of bovine lens alpha A-crystallin

International Journal of Biological Macromolecules
P N FarnsworthK Singh

Abstract

In absence of 3D structures for alpha-crystallin subunits, alpha A and alpha B, we utilized a number of experimental and molecular modeling techniques to generate working 3D models of these polypeptides (Farnsworth et al., 1994. In Molecular Modeling: From Virtual Tools to Real Problems (Eds. Kumosinski, T.F. and Liebman, M.N.) ACS Symposium Series 576, Ch. 9:123-134, 1994, ACS Books, Washington DC). The refinement of the initial bovine alpha A model was achieved using a more accurate estimation of secondary structure by new/updated methods for analyzing the far UV-CD spectra and by neural network secondary structure predictions in combination with database searches. The spectroscopic study reveals that alpha-crystallin is not an all beta-sheet protein but contains approximately 17% alpha-helices, approximately 33% beta-structures and approximately 50% turns and coils. The refinement of the alpha A structure results in an elongate, asymmetric amphipathic molecule. The hydrophobic N-terminal domain imparts the driving force for subunit aggregation while the more flexible, polar C-terminal domain imparts aggregate solubility. In our quaternary structure of the aggregate, the monomer is the minimal cooperative subunit. In bovine a...Continue Reading

References

Feb 9, 1978·Nature·W Gilbert
Dec 30, 1992·Biochemical and Biophysical Research Communications·M C ReddyP N Farnsworth
Apr 6, 1992·Biochimica Et Biophysica Acta·K B MerckW W De Jong
Dec 18, 1991·European Journal of Biochemistry·S P BhatL Ding
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·F X LuI Chaiken
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R KlemenzA Aoyama
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1988·Annual Review of Biochemistry·G J Wistow, J Piatigorsky
Sep 28, 1987·FEBS Letters·R C Augusteyn, J F Koretz
Oct 1, 1986·European Journal of Biochemistry·C E VoorterW W de Jong
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T D Ingolia, E A Craig
Jan 6, 1981·Biochemistry·S W Provencher, J Glöckner
Aug 1, 1995·Experimental Eye Research·B Groth-VasselliP N Farnsworth
Jan 17, 1995·Biochemistry·Z T FarahbakhshW L Hubbell
Feb 16, 1994·Biochimica Et Biophysica Acta·J A CarverR J Truscott
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·M Kantorow, J Piatigorsky
Dec 6, 1995·Biochimica Et Biophysica Acta·I V DudichT Korpela
Apr 1, 1996·International Journal of Biological Macromolecules·K SinghP N Farnsworth
Dec 1, 1996·International Journal of Biological Macromolecules·K SinghP N Farnsworth
May 9, 1997·The Journal of Biological Chemistry·M R LerouxE P Candido
Jul 3, 1998·International Journal of Biological Macromolecules·R H SmuldersW W de Jong
Jul 3, 1998·International Journal of Biological Macromolecules·K SinghP N Farnsworth

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Citations

Jun 10, 2000·European Journal of Biochemistry·J VanhoudtJ Clauwaert
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Franz Narberhaus
Oct 17, 2002·Cell Stress & Chaperones·Nicole Mounier, André-Patrick Arrigo
Jul 27, 2015·Cellular and Molecular Life Sciences : CMLS·Erin Thornell, Andrew Aquilina
Jan 1, 2003·Biochemical and Biophysical Research Communications·Asmat Salim, Zafar H Zaidi
Jan 28, 2006·Biochemical and Biophysical Research Communications·Rita GalántaiRaymund Machovich
Jan 20, 2005·Experimental Eye Research·P Farnsworth, Kamalendra Singh
Feb 17, 2001·Journal of Molecular Recognition : JMR·A P Demchenko
Jan 11, 2000·Eye·J HorwitzP L Stewart
Mar 30, 2011·Proteins·Tue RasmussenMarina R Kasimova
Jul 17, 1999·Biochimica Et Biophysica Acta·M BloemendalW C Johnson
Apr 12, 2000·Biochemistry·J VanhoudtJ Clauwaert

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