Refinement of a thyroid cultured cell system used in clinically relevant bioassays.

Cytotechnology
F S Ambesi-ImpiombatoF Curcio

Abstract

Thyroid cultured cells are now used worldwide in clinical bioassays of TSH and of thyroid autoantibodies. Having originally developed the thyroid cell cultures (Ambesi-Impiombato et al. 1980) from rat glands in our laboratory, we now aim to improve the system, moving in two directions: a) TSH-independent mutants have been produced and characterized, which can be used in clinical bioassays without "starvation" from the hormone. b) Human cultures have been attempted using our experience with rat cells, as well as innovative strategies. Preliminary results now indicate that human normal differentiated cells may be available for clinical studies in vitro, when species-specific differences may be critical.

References

Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·F S Ambesi-ImpiombatoH G Coon
Oct 27, 1983·The New England Journal of Medicine·W A ValenteL D Kohn
Jun 1, 1984·The Journal of Endocrinology·S P BideyN J Marshall
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·F CurcioH G Coon

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Citations

Jul 1, 1997·International Journal of Food Sciences and Nutrition·M J Msolla, J L Kinabo

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