Refinement of the conformation of UDP-galactose bound to galactosyltransferase using the STD NMR intensity-restrained CORCEMA optimization

Journal of the American Chemical Society
V JayalakshmiN Rama Krishna

Abstract

The STD NMR technique has originally been described as a tool for screening large compound libraries to identify the lead compounds that are specific to target proteins of interest. The application of this technique in the qualitative epitope mapping of ligands weakly binding to proteins, virus capsid shells, and nucleic acids has also been described. Here we describe the application of the STD NMR intensity-restrained CORCEMA optimization (SICO) procedure for refining the bound conformation of UDP-galactose in galactosyltransferase complex using STD NMR intensities recorded at 500 MHz as the experimental constraints. A comparison of the SICO structure for the bound UDP-galactose in solution with that in the crystal structure for this complex shows some differences in ligand torsion angles and V253 side-chain orientation in the protein. This work describes the first application of an STD NMR intensity-restrained CORCEMA optimization procedure for refining the torsion angles of a bound ligand structure. This method is likely to be useful in structure-based drug design programs since most initial lead compounds generally exhibit weak affinity (millimolar to micromolar) to target proteins of pharmaceutical interest, and the bound ...Continue Reading

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Citations

Sep 29, 2011·European Biophysics Journal : EBJ·Jesus Angulo, Pedro M Nieto
May 12, 2007·Glycoconjugate Journal·Caroline ClavelJesús Jiménez-Barbero
Apr 18, 2013·Analytical and Bioanalytical Chemistry·Olivier CalaIsabelle Krimm
Oct 3, 2006·Journal of Biomolecular NMR·Manuel Martín-PastorJesús Jiménez-Barbero
Jan 3, 2012·Drug Discovery Today·Anirban BhuniaSubhrangsu Chatterjee
Nov 5, 2014·Frontiers in Immunology·Inka Brockhausen
Aug 5, 2016·Molecular Informatics·Mattia SturleseStefano Moro
Aug 23, 2008·Chembiochem : a European Journal of Chemical Biology·Jesús AnguloPedro M Nieto
Apr 14, 2011·Natural Product Reports·Luis P CalleJesús Jiménez-Barbero
Apr 19, 2011·Chembiochem : a European Journal of Chemical Biology·Virginia RoldósJesús Jiménez-Barbero
Feb 27, 2018·Journal of Chemical Information and Modeling·Joana MagalhãesGabriele Costantino

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