Refining a model electron-density map via the Phantom Derivative method

Acta Crystallographica. Section D, Biological Crystallography
Maria Cristina BurlaGiampiero Polidori

Abstract

The Phantom Derivative (PhD) method [Giacovazzo (2015), Acta Cryst. A71, 483-512] has recently been described for ab initio and non-ab initio phasing. It is based on the random generation of structures with the same unit cell and the same space group as the target structure (called ancil structures), which are used to create derivatives devoid of experimental diffraction amplitudes. In this paper, the non-ab initio variant of the method was checked using phase sets obtained by molecular-replacement techniques as a starting point for phase extension and refinement. It has been shown that application of PhD is able to extend and refine phases in a way that is competitive with other electron-density modification techniques.

References

Sep 18, 1999·Acta Crystallographica. Section D, Biological Crystallography·K Cowtan
Jul 1, 1993·Acta Crystallographica. Section D, Biological Crystallography·L S Refaat, M M Woolfson
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Apr 23, 2005·Acta Crystallographica. Section A, Foundations of Crystallography·Rocco CaliandroDritan Siliqi
Aug 25, 2006·Acta Crystallographica. Section D, Biological Crystallography·Kevin Cowtan
Jan 28, 2009·Acta Crystallographica. Section D, Biological Crystallography·Rhiju Das, David Baker
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Garib N MurshudovAlexei A Vagin
May 23, 2013·Acta Crystallographica. Section D, Biological Crystallography·Benedetta CarrozziniAnnamaria Mazzone
Jul 10, 2014·Acta Crystallographica. Section D, Biological Crystallography·Rocco CaliandroAnnamaria Mazzone
Sep 1, 2015·Acta Crystallographica. Section A, Foundations and Advances·Carmelo Giacovazzo
Sep 4, 2015·Acta Crystallographica. Section D, Biological Crystallography·Benedetta CarrozziniAnnamaria Mazzone

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Citations

Apr 7, 2016·Acta Crystallographica. Section D, Structural Biology·Benedetta CarrozziniCarmelo Giacovazzo
Apr 28, 2017·Acta Crystallographica. Section A, Foundations and Advances·Maria Cristina BurlaGiampiero Polidori
Nov 3, 2017·Acta Crystallographica. Section D, Structural Biology·Maria Cristina BurlaGiampiero Polidori
Dec 24, 2018·Acta Crystallographica. Section A, Foundations and Advances·Carmelo Giacovazzo

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