Refining a steroidogenic model: an analysis of RNA-seq datasets from insect prothoracic glands

BMC Genomics
Panagiotis MoulosSkarlatos G Dedos

Abstract

The prothoracic gland (PG), the principal steroidogenic organ of insects, has been proposed as a model for steroid hormone biosynthesis and regulation. To validate the robustness of the model, we present an analysis of accumulated transcriptomic data from PGs of two model species, Drosophila melanogaster and Bombyx mori. We identify that the common core components of the model in both species are encoded by nine genes. Five of these are Halloween genes whose expression differs substantially between the PGs of these species. We conclude that the PGs can be a model for steroid hormone synthesis and regulation within the context of mitochondrial cholesterol transport and steroid biosynthesis but beyond these core mechanisms, gene expression in insect PGs is too diverse to fit in a context-specific model and should be analysed within a species-specific framework.

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Citations

Sep 21, 2020·Current Opinion in Insect Science·Xueyang PanMichael B O'Connor
Jul 9, 2021·Frontiers in Endocrinology·Donald L Mykles

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Datasets Mentioned

BETA
DRA002282

Methods Mentioned

BETA
RNA-seq
transgenic
gene knockdown
acetylation
PCR
electrophoresis

Software Mentioned

NBPSeq
Orth
DESeq
baySeq
REDfly
Prottest
Cocoa
MrBayes
tBLASTN
Bowtie2

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