Refining cell-based assay to detect MOG-IgG in patients with central nervous system inflammatory diseases.

Multiple Sclerosis and Related Disorders
Yeseul KimHo Jin Kim

Abstract

Given that the spectrum of myelin oligodendrocyte glycoprotein immunoglobulin G (MOG-IgG) associated disease is yet to be fully defined, development of sensitive and highly specific assays to identify MOG-IgG is crucial to precisely define the clinical phenotypes, disease courses and prognosis to describe the full spectrum of MOG-IgG associated diseases. Here, we aim to validate a new in-house live cell-based assay (CBA) for screening MOG-IgG in patients with central nervous system inflammatory diseases. We generated a full length MOG transfected HEK293 stable cell line using pIRES2-eGFP vector. Sera from 355 patients with central nervous system inflammatory diseases and 25 healthy individuals were evaluated for MOG-IgG seropositivity using in-house cell-based immunofluorescence assay (CBA-IF). The specificity of IgG (H + L) and IgG1-Fc secondary antibodies as well as IgM binding were determined by cell-based flow cytometry (CBA-FACS). The optimal cut-offs for determining seropositivity in CBA-FACS were calculated and the concordance of CBA-IF score and CBA-FACS was studied. The results of our CBA-IF were compared with the Oxford CBA-IF. 11.5% (41/355) of patients were seropositive for MOG-IgG and had clinical phenotypes that w...Continue Reading

Citations

Aug 12, 2020·Multiple Sclerosis : Clinical and Laboratory Research·Jae-Won HyunHo Jin Kim
Apr 2, 2020·Multiple Sclerosis : Clinical and Laboratory Research·Jae-Won HyunHo Jin Kim
Jun 26, 2020·Frontiers in Neurology·Jae-Won HyunHo Jin Kim
Oct 17, 2020·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Amanda MarchionattiDouglas Kazutoshi Sato
Nov 17, 2020·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·Thaís ArmangueUNKNOWN E.U. paediatric MOG consortium
Aug 22, 2021·Lancet Neurology·Romain MarignierBernhard Hemmer

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