Refining the phylum Chlorobi by resolving the phylogeny and metabolic potential of the representative of a deeply branching, uncultivated lineage

The ISME Journal
Jennifer HirasSteven W Singer

Abstract

Recent studies have expanded the phylum Chlorobi, demonstrating that the green sulfur bacteria (GSB), the original cultured representatives of the phylum, are a part of a broader lineage whose members have more diverse metabolic capabilities that overlap with members of the phylum Bacteroidetes. The 16S rRNA gene of an uncultivated clone, OPB56, distantly related to the phyla Chlorobi and Bacteroidetes, was recovered from Obsidian Pool in Yellowstone National Park; however, the detailed phylogeny and function of OPB56 and related clones have remained unknown. Culturing of thermophilic bacterial consortia from compost by adaptation to grow on ionic-liquid pretreated switchgrass provided a consortium in which one of the most abundant members, NICIL-2, clustered with OPB56-related clones. Phylogenetic analysis using the full-length 16S rRNA gene from NICIL-2 demonstrated that it was part of a monophyletic clade, referred to as OPB56, distinct from the Bacteroidetes and Chlorobi. A near complete draft genome (>95% complete) was recovered from metagenomic data from the culture adapted to grow on ionic-liquid pretreated switchgrass using an automated binning algorithm, and this genome was used for marker gene-based phylogenetic analy...Continue Reading

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Citations

Mar 6, 2018·Annual Review of Plant Biology·Vera ThielDonald A Bryant
Jul 10, 2018·Applied Microbiology and Biotechnology·Hao ZhouAnthony G Hay
Oct 5, 2018·Frontiers in Microbiology·Joline El-ChakhtouraJohannes S Vrouwenvelder
Oct 26, 2018·Scientific Reports·Corina ItcusCristina Purcarea
Jan 17, 2019·MBio·Gerald L Newton, Mamta Rawat
Jul 22, 2017·Frontiers in Microbiology·Katharine J ThompsonSean A Crowe

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